LOCUS BBa_S03780 1180 bp DNA linear 28-May-2024 DEFINITION hixC-RFP2-RBS-GFP1-hixC-GFP2-TT ACCESSION BBa_S03780 VERSION BBa_S03780.1 FEATURES Location/Qualifiers misc_feature 1..26 /label=BBa_J44000 misc_feature 35..35 /label=extra base (avoid frameshift) CDS 36..248 /label=Continue coding sequence misc_feature 249..253 /label=(two in tandem) CDS 35..254 /label=BBa_I715023 misc_feature 267..270 /label=conserved RBS 263..274 /label=BBa_B0034 misc_feature 281..283 /label=Start Codon CDS 281..751 /label=Coding Amino Portion GFP (aka GFP1) misc_feature 281..751 /label=BBa_I715019 misc_feature 760..785 /label=BBa_J44000 misc_feature 794..794 /label=extra base to maintain reading frame CDS 794..1037 /label=Coding Carboxyl half of GFP (aka GFP2) misc_feature 1038..1043 /label=Stop Codons (two in tandem) misc_feature 794..1043 /label=BBa_I715020 stem_loop 1063..1106 /label=stem_loop misc_feature 1052..1131 /label=BBa_B0010 terminator 1052..1131 /label=BBa_B0010 stem_loop 1147..1166 /label=T7 TE misc_feature 1173..1173 /label=stop polyA_site 1167..1180 /label=polya misc_feature 1140..1180 /label=BBa_B0012 terminator 1140..1180 /label=BBa_B0012 ORIGIN 1 ttatcaaaaa ccatggtttt tgataatact agagtggtgc tctgaaaggt gaaatcaaaa 61 tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg 121 ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct 181 cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca 241 ccggtgctta ataatactag agaaagagga gaaatactag atgcgtaaag gagaagaact 301 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt 361 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat 421 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg 481 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc 541 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa 601 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg 661 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc 721 acacaatgta tacatcatgg cagacaaaca atactagagt tatcaaaaac catggttttt 781 gataatacta gagtaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag 841 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg 901 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg 961 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca 1021 tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag 1081 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 1141 cacactggct caccttcggg tgggcctttc tgcgtttata //