LOCUS       BBa_S03786              1613 bp    DNA     linear       02-May-2025
DEFINITION  hixC-RFP2-RBS-GFP1-hixC-RFP2-TT-hixC-GFP2-TT-hixC
ACCESSION   BBa_S03786
VERSION     BBa_S03786.1  
FEATURES             Location/Qualifiers
     misc_feature    1..26
                     /label=BBa_J44000
     misc_feature    35..35
                     /label=extra base (avoid frameshift)
     CDS             36..248
                     /label=Continue coding sequence
     misc_feature    249..253
                     /label=(two in tandem)
     CDS             35..254
                     /label=BBa_I715023
     misc_feature    267..270
                     /label=conserved
     RBS             263..274
                     /label=BBa_B0034
     misc_feature    281..283
                     /label=Start Codon
     CDS             281..751
                     /label=Coding Amino Portion GFP (aka GFP1)
     misc_feature    281..751
                     /label=BBa_I715019
     misc_feature    760..785
                     /label=BBa_J44000
     misc_feature    794..794
                     /label=extra base (avoid frameshift)
     CDS             795..1007
                     /label=Continue coding sequence
     misc_feature    1008..1012
                     /label=(two in tandem)
     CDS             794..1013
                     /label=BBa_I715023
     stem_loop       1033..1076
                     /label=stem_loop
     misc_feature    1022..1101
                     /label=BBa_B0010
     terminator      1022..1101
                     /label=BBa_B0010
     stem_loop       1117..1136
                     /label=T7 TE
     misc_feature    1143..1143
                     /label=stop
     polyA_site      1137..1150
                     /label=polya
     misc_feature    1110..1150
                     /label=BBa_B0012
     terminator      1110..1150
                     /label=BBa_B0012
     misc_feature    1159..1184
                     /label=BBa_J44000
     misc_feature    1193..1193
                     /label=extra base to maintain reading frame
     CDS             1193..1436
                     /label=Coding Carboxyl half of GFP (aka GFP2)
     misc_feature    1437..1442
                     /label=Stop Codons (two in tandem)
     misc_feature    1193..1442
                     /label=BBa_I715020
     stem_loop       1462..1505
                     /label=stem_loop
     misc_feature    1451..1530
                     /label=BBa_B0010
     terminator      1451..1530
                     /label=BBa_B0010
     stem_loop       1546..1565
                     /label=T7 TE
     misc_feature    1572..1572
                     /label=stop
     polyA_site      1566..1579
                     /label=polya
     misc_feature    1539..1579
                     /label=BBa_B0012
     terminator      1539..1579
                     /label=BBa_B0012
     misc_feature    1588..1613
                     /label=BBa_J44000
ORIGIN
        1 ttatcaaaaa ccatggtttt tgataatact agagtggtgc tctgaaaggt gaaatcaaaa
       61 tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg
      121 ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct
      181 cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca
      241 ccggtgctta ataatactag agaaagagga gaaatactag atgcgtaaag gagaagaact
      301 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      361 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
      421 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg
      481 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc
      541 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
      601 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
      661 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
      721 acacaatgta tacatcatgg cagacaaaca atactagagt tatcaaaaac catggttttt
      781 gataatacta gagtggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg
      841 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc
      901 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca
      961 tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taatactaga
     1021 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1081 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1141 tgcgtttata tactagagtt atcaaaaacc atggtttttg ataatactag agtaagaatg
     1201 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
     1261 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     1321 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     1381 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
     1441 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1501 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1561 gggcctttct gcgtttatat actagagtta tcaaaaacca tggtttttga taa
//