LOCUS       BBa_S03787              1613 bp    DNA     linear       29-Nov-2024
DEFINITION  hixC-GFP2-TT-hixC-RFP2-RBS-GFP1-hixC-RFP2-TT-hixC
ACCESSION   BBa_S03787
VERSION     BBa_S03787.1  
FEATURES             Location/Qualifiers
     misc_feature    1..26
                     /label=BBa_J44000
     misc_feature    35..35
                     /label=extra base to maintain reading frame
     CDS             35..278
                     /label=Coding Carboxyl half of GFP (aka GFP2)
     misc_feature    279..284
                     /label=Stop Codons (two in tandem)
     misc_feature    35..284
                     /label=BBa_I715020
     stem_loop       304..347
                     /label=stem_loop
     misc_feature    293..372
                     /label=BBa_B0010
     terminator      293..372
                     /label=BBa_B0010
     stem_loop       388..407
                     /label=T7 TE
     misc_feature    414..414
                     /label=stop
     polyA_site      408..421
                     /label=polya
     misc_feature    381..421
                     /label=BBa_B0012
     terminator      381..421
                     /label=BBa_B0012
     misc_feature    430..455
                     /label=BBa_J44000
     misc_feature    464..464
                     /label=extra base (avoid frameshift)
     CDS             465..677
                     /label=Continue coding sequence
     misc_feature    678..682
                     /label=(two in tandem)
     CDS             464..683
                     /label=BBa_I715023
     misc_feature    696..699
                     /label=conserved
     RBS             692..703
                     /label=BBa_B0034
     misc_feature    710..712
                     /label=Start Codon
     CDS             710..1180
                     /label=Coding Amino Portion GFP (aka GFP1)
     misc_feature    710..1180
                     /label=BBa_I715019
     misc_feature    1189..1214
                     /label=BBa_J44000
     misc_feature    1223..1223
                     /label=extra base (avoid frameshift)
     CDS             1224..1436
                     /label=Continue coding sequence
     misc_feature    1437..1441
                     /label=(two in tandem)
     CDS             1223..1442
                     /label=BBa_I715023
     stem_loop       1462..1505
                     /label=stem_loop
     misc_feature    1451..1530
                     /label=BBa_B0010
     terminator      1451..1530
                     /label=BBa_B0010
     stem_loop       1546..1565
                     /label=T7 TE
     misc_feature    1572..1572
                     /label=stop
     polyA_site      1566..1579
                     /label=polya
     misc_feature    1539..1579
                     /label=BBa_B0012
     terminator      1539..1579
                     /label=BBa_B0012
     misc_feature    1588..1613
                     /label=BBa_J44000
ORIGIN
        1 ttatcaaaaa ccatggtttt tgataatact agagtaagaa tggaatcaaa gttaacttca
       61 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
      121 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
      181 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
      241 ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag agccaggcat
      301 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      361 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      421 atactagagt tatcaaaaac catggttttt gataatacta gagtggtgct ctgaaaggtg
      481 aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca
      541 cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg
      601 acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc
      661 gtcactccac cggtgcttaa taatactaga gaaagaggag aaatactaga tgcgtaaagg
      721 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      781 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      841 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      901 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
      961 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
     1021 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
     1081 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
     1141 ctataactca cacaatgtat acatcatggc agacaaacaa tactagagtt atcaaaaacc
     1201 atggtttttg ataatactag agtggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
     1261 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
     1321 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
     1381 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
     1441 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1501 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1561 gggcctttct gcgtttatat actagagtta tcaaaaacca tggtttttga taa
//