BBa_S03791
type: ComponentDefinition
persistent identity: BBa S03791
display id: BBa_S03791
version: 1
was generated by: iGEM to SBOL conversion
description: C0012:S03790
created: 2007-08-28T11:00:00Z
modified: 2015-05-08T01:14:30Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 2051
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: CHEN Daizhuo
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0012
component: BBa_S03790
sequence annotation: BBa_C0012
sequence annotation: BBa_S03790
sequence: https://synbiohub.org/public/igem/BBa_S03791_sequence/1

BBa_C0012
type: Component
persistent identity: component2253046
display id: component2253046
version: 1
top level: BBa_S03791
definition: lacI
access: public

BBa_S03790
type: Component
persistent identity: component2253066
display id: component2253066
version: 1
top level: BBa_S03791
definition: BBa_S03790
access: public

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2253046
display id: annotation2253046
version: 1
top level: BBa_S03791
location: https://synbiohub.org/public/igem/BBa_S03791/annotation2253046/range2253046/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_S03791/annotation2253046/range2253046/1
type: Range
persistent identity: range2253046
display id: range2253046
version: 1
top level: BBa_S03791
direction: direction/forward
start: 1
end: 1128
orientation: inline

BBa_S03790
type: SequenceAnnotation
persistent identity: annotation2253066
display id: annotation2253066
version: 1
top level: BBa_S03791
location: https://synbiohub.org/public/igem/BBa_S03791/annotation2253066/range2253066/1
component: BBa_S03790

https://synbiohub.org/public/igem/BBa_S03791/annotation2253066/range2253066/1
type: Range
persistent identity: range2253066
display id: range2253066
version: 1
top level: BBa_S03791
direction: direction/reverse
start: 1160
end: 3411
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

BBa_S03790
type: ComponentDefinition
persistent identity: BBa S03790
display id: BBa_S03790
version: 1
was generated by: iGEM to SBOL conversion
description: E0040:S03789
created: 2007-08-28T11:00:00Z
modified: 2015-05-08T01:14:30Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 2051
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: CHEN Daizhuo
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_E0040
component: BBa_S03789
sequence annotation: BBa_E0040
sequence annotation: BBa_S03789
sequence: https://synbiohub.org/public/igem/BBa_S03790_sequence/1

BBa_E0040
type: Component
persistent identity: component2248789
display id: component2248789
version: 1
top level: BBa_S03790
definition: GFP
access: public

BBa_S03789
type: Component
persistent identity: component2248803
display id: component2248803
version: 1
top level: BBa_S03790
definition: BBa_S03789
access: public

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2248789
display id: annotation2248789
version: 1
top level: BBa_S03790
location: https://synbiohub.org/public/igem/BBa_S03790/annotation2248789/range2248789/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_S03790/annotation2248789/range2248789/1
type: Range
persistent identity: range2248789
display id: range2248789
version: 1
top level: BBa_S03790
direction: direction/reverse
start: 1
end: 720
orientation: inline

BBa_S03789
type: SequenceAnnotation
persistent identity: annotation2248803
display id: annotation2248803
version: 1
top level: BBa_S03790
location: https://synbiohub.org/public/igem/BBa_S03790/annotation2248803/range2248803/1
component: BBa_S03789

https://synbiohub.org/public/igem/BBa_S03790/annotation2248803/range2248803/1
type: Range
persistent identity: range2248803
display id: range2248803
version: 1
top level: BBa_S03790
direction: direction/reverse
start: 727
end: 2252
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_S03789
type: ComponentDefinition
persistent identity: BBa S03789
display id: BBa_S03789
version: 1
was generated by: iGEM to SBOL conversion
description: C0051:S03788
created: 2007-08-28T11:00:00Z
modified: 2015-05-08T01:14:30Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 2051
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: CHEN Daizhuo
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_C0051
component: BBa_S03788
sequence annotation: BBa_C0051
sequence annotation: BBa_S03788
sequence: https://synbiohub.org/public/igem/BBa_S03789_sequence/1

BBa_C0051
type: Component
persistent identity: component2245635
display id: component2245635
version: 1
top level: BBa_S03789
definition: cI lam
access: public

BBa_S03788
type: Component
persistent identity: component2245644
display id: component2245644
version: 1
top level: BBa_S03789
definition: BBa_S03788
access: public

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2245635
display id: annotation2245635
version: 1
top level: BBa_S03789
location: https://synbiohub.org/public/igem/BBa_S03789/annotation2245635/range2245635/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_S03789/annotation2245635/range2245635/1
type: Range
persistent identity: range2245635
display id: range2245635
version: 1
top level: BBa_S03789
direction: direction/reverse
start: 1
end: 775
orientation: inline

BBa_S03788
type: SequenceAnnotation
persistent identity: annotation2245644
display id: annotation2245644
version: 1
top level: BBa_S03789
location: https://synbiohub.org/public/igem/BBa_S03789/annotation2245644/range2245644/1
component: BBa_S03788

https://synbiohub.org/public/igem/BBa_S03789/annotation2245644/range2245644/1
type: Range
persistent identity: range2245644
display id: range2245644
version: 1
top level: BBa_S03789
direction: direction/reverse
start: 784
end: 1526
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

BBa_S03788
type: ComponentDefinition
persistent identity: BBa S03788
display id: BBa_S03788
version: 1
was generated by: iGEM to SBOL conversion
description: I714888:C0056
created: 2007-08-28T11:00:00Z
modified: 2015-05-08T01:14:30Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 2051
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: CHEN Daizhuo
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_I714888
component: BBa_C0056
sequence annotation: BBa_I714888
sequence annotation: BBa_C0056
sequence: https://synbiohub.org/public/igem/BBa_S03788_sequence/1

BBa_I714888
type: Component
persistent identity: component1942859
display id: component1942859
version: 1
top level: BBa_S03788
definition: BBa_I714888
access: public

BBa_C0056
type: Component
persistent identity: component1942862
display id: component1942862
version: 1
top level: BBa_S03788
definition: BBa_C0056
access: public

BBa_I714888
type: SequenceAnnotation
persistent identity: annotation1942859
display id: annotation1942859
version: 1
top level: BBa_S03788
location: https://synbiohub.org/public/igem/BBa_S03788/annotation1942859/range1942859/1
component: BBa_I714888

https://synbiohub.org/public/igem/BBa_S03788/annotation1942859/range1942859/1
type: Range
persistent identity: range1942859
display id: range1942859
version: 1
top level: BBa_S03788
direction: direction/reverse
start: 1
end: 101
orientation: inline

BBa_C0056
type: SequenceAnnotation
persistent identity: annotation1942862
display id: annotation1942862
version: 1
top level: BBa_S03788
location: https://synbiohub.org/public/igem/BBa_S03788/annotation1942862/range1942862/1
component: BBa_C0056

https://synbiohub.org/public/igem/BBa_S03788/annotation1942862/range1942862/1
type: Range
persistent identity: range1942862
display id: range1942862
version: 1
top level: BBa_S03788
direction: direction/reverse
start: 108
end: 743
orientation: inline

BBa_C0056
type: ComponentDefinition
persistent identity: BBa C0056
display id: BBa_C0056
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from phage 434 (no LVA)
created: 2004-09-01T11:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Removed LVA tag from BBa_C0052
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Removed LVA tag from BBa_C0052 via PCR. In pSB1AK3 currently.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: cI 434
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_C0056_sequence/1

cI 434
type: SequenceAnnotation
persistent identity: annotation1063931
display id: annotation1063931
version: 1
top level: BBa_C0056
location: https://synbiohub.org/public/igem/BBa_C0056/annotation1063931/range1063931/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0056/annotation1063931/range1063931/1
type: Range
persistent identity: range1063931
display id: range1063931
version: 1
top level: BBa_C0056
direction: direction/forward
start: 1
end: 636
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1063930
display id: annotation1063930
version: 1
top level: BBa_C0056
location: https://synbiohub.org/public/igem/BBa_C0056/annotation1063930/range1063930/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_C0056/annotation1063930/range1063930/1
type: Range
persistent identity: range1063930
display id: range1063930
version: 1
top level: BBa_C0056
direction: direction/forward
start: 631
end: 636
orientation: inline

BBa_I714888
type: ComponentDefinition
persistent identity: BBa I714888
display id: BBa_I714888
version: 1
was generated by: iGEM to SBOL conversion
description: double direction promoter (cI/cI434)
created: 2007-08-28T11:00:00Z
modified: 2015-08-31T04:07:48Z
mutable provenance: n/a
owned by: james
owned by: myers
mutable description: double direction promoter (cI/cI434)
discontinued: true
dominant: false
experience: experience/Fails
group u list: _142_
m user id: 0
owner id: 2051
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: n/a
star: false
creator: CHEN Daizhuo
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: pL
sequence annotation: pRM
sequence annotation: cI 434 OR1
sequence: https://synbiohub.org/public/igem/BBa_I714888_sequence/1

pL
type: SequenceAnnotation
persistent identity: annotation1942853
display id: annotation1942853
version: 1
top level: BBa_I714888
location: https://synbiohub.org/public/igem/BBa_I714888/annotation1942853/range1942853/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I714888/annotation1942853/range1942853/1
type: Range
persistent identity: range1942853
display id: range1942853
version: 1
top level: BBa_I714888
direction: direction/reverse
start: 21
end: 25
orientation: inline

pRM
type: SequenceAnnotation
persistent identity: annotation1942854
display id: annotation1942854
version: 1
top level: BBa_I714888
location: https://synbiohub.org/public/igem/BBa_I714888/annotation1942854/range1942854/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I714888/annotation1942854/range1942854/1
type: Range
persistent identity: range1942854
display id: range1942854
version: 1
top level: BBa_I714888
direction: direction/forward
start: 31
end: 35
orientation: inline

cI 434 OR1
type: SequenceAnnotation
persistent identity: annotation1942851
display id: annotation1942851
version: 1
top level: BBa_I714888
location: https://synbiohub.org/public/igem/BBa_I714888/annotation1942851/range1942851/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_I714888/annotation1942851/range1942851/1
type: Range
persistent identity: range1942851
display id: range1942851
version: 1
top level: BBa_I714888
direction: direction/forward
start: 56
end: 71
orientation: inline

cI 434 OR1
type: SequenceAnnotation
persistent identity: annotation1942852
display id: annotation1942852
version: 1
top level: BBa_I714888
location: https://synbiohub.org/public/igem/BBa_I714888/annotation1942852/range1942852/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_I714888/annotation1942852/range1942852/1
type: Range
persistent identity: range1942852
display id: range1942852
version: 1
top level: BBa_I714888
direction: direction/forward
start: 86
end: 101
orientation: inline

https://synbiohub.org/public/igem/BBa_S03791_sequence/1
type: Sequence
persistent identity: BBa S03791 sequence
display id: BBa_S03791_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03790_sequence/1
type: Sequence
persistent identity: BBa S03790 sequence
display id: BBa_S03790_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttacaagaaagtttgttagatttaacgtatgtacaagaaagtttgtttactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03789_sequence/1
type: Sequence
persistent identity: BBa S03789 sequence
display id: BBa_S03789_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttacaagaaagtttgttagatttaacgtatgtacaagaaagtttgtttactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03788_sequence/1
type: Sequence
persistent identity: BBa S03788 sequence
display id: BBa_S03788_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttacaagaaagtttgttagatttaacgtatgtacaagaaagtttgtttactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0056_sequence/1
type: Sequence
persistent identity: BBa C0056 sequence
display id: BBa_C0056_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I714888_sequence/1
type: Sequence
persistent identity: BBa I714888 sequence
display id: BBa_I714888_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttacaagaaagtttgttagatttaacgtatgtacaagaaagtttgtt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z