LOCUS       BBa_S03803               986 bp    DNA     linear       01-Jun-2024
DEFINITION  B0012:I13522
ACCESSION   BBa_S03803
VERSION     BBa_S03803.1  
FEATURES             Location/Qualifiers
     stem_loop       8..27
                     /label=T7 TE
     misc_feature    34..34
                     /label=stop
     polyA_site      28..41
                     /label=polya
     misc_feature    1..41
                     /label=BBa_B0012
     terminator      1..41
                     /label=BBa_B0012
     misc_feature    50..68
                     /label=TetR 1
     misc_feature    50..103
                     /label=BBa_R0040
     promoter        69..74
                     /label=-35
     misc_feature    75..93
                     /label=TetR 2
     promoter        92..97
                     /label=-10
     promoter        50..103
                     /label=BBa_R0040
     misc_feature    116..119
                     /label=conserved
     RBS             112..123
                     /label=BBa_B0034
     CDS             130..849
                     /label=GFP protein
     CDS             130..849
                     /label=BBa_E0040
     stem_loop       869..912
                     /label=stem_loop
     misc_feature    858..937
                     /label=BBa_B0010
     terminator      858..937
                     /label=BBa_B0010
     stem_loop       953..972
                     /label=T7 TE
     misc_feature    979..979
                     /label=stop
     polyA_site      973..986
                     /label=polya
     misc_feature    946..986
                     /label=BBa_B0012
     terminator      946..986
                     /label=BBa_B0012
ORIGIN
        1 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt ccctatcagt
       61 gatagagatt gacatcccta tcagtgatag agatactgag cactactaga gaaagaggag
      121 aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
      181 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
      241 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
      301 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat
      361 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
      421 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
      481 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
      541 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
      601 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
      661 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
      721 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
      781 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
      841 aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      901 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      961 ttcgggtggg cctttctgcg tttata
//