LOCUS       BBa_S03807              1857 bp    DNA     linear       23-Dec-2024
DEFINITION  S03802:S03803
ACCESSION   BBa_S03807
VERSION     BBa_S03807.1  
FEATURES             Location/Qualifiers
     CDS             4..711
                     /label=cI lambda
     misc_feature    712..744
                     /label=LVA
     misc_feature    751..775
                     /label=Help:Barcodes
     CDS             1..775
                     /label=BBa_C0051
     stem_loop       795..838
                     /label=stem_loop
     misc_feature    784..863
                     /label=BBa_B0010
     terminator      784..863
                     /label=BBa_B0010
     misc_feature    1..863
                     /label=BBa_S03802
     stem_loop       879..898
                     /label=T7 TE
     misc_feature    905..905
                     /label=stop
     polyA_site      899..912
                     /label=polya
     misc_feature    872..912
                     /label=BBa_B0012
     terminator      872..912
                     /label=BBa_B0012
     misc_feature    921..939
                     /label=TetR 1
     misc_feature    921..974
                     /label=BBa_R0040
     promoter        940..945
                     /label=-35
     misc_feature    946..964
                     /label=TetR 2
     promoter        963..968
                     /label=-10
     promoter        921..974
                     /label=BBa_R0040
     misc_feature    987..990
                     /label=conserved
     RBS             983..994
                     /label=BBa_B0034
     CDS             1001..1720
                     /label=GFP protein
     CDS             1001..1720
                     /label=BBa_E0040
     stem_loop       1740..1783
                     /label=stem_loop
     misc_feature    1729..1808
                     /label=BBa_B0010
     terminator      1729..1808
                     /label=BBa_B0010
     stem_loop       1824..1843
                     /label=T7 TE
     misc_feature    1850..1850
                     /label=stop
     polyA_site      1844..1857
                     /label=polya
     misc_feature    1817..1857
                     /label=BBa_B0012
     terminator      1817..1857
                     /label=BBa_B0012
     misc_feature    872..1857
                     /label=BBa_S03803
ORIGIN
        1 atgagcacaa aaaagaaacc attaacacaa gagcagcttg aggacgcacg tcgccttaaa
       61 gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc aggaatctgt cgcagacaag
      121 atggggatgg ggcagtcagg cgttggtgct ttatttaatg gcatcaatgc attaaatgct
      181 tataacgccg cattgcttgc aaaaattctc aaagttagcg ttgaagaatt tagcccttca
      241 atcgccagag aaatctacga gatgtatgaa gcggttagta tgcagccgtc acttagaagt
      301 gagtatgagt accctgtttt ttctcatgtt caggcaggga tgttctcacc tgagcttaga
      361 acctttacca aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct
      421 gcattctggc ttgaggttga aggtaattcc atgaccgcac caacaggctc caagccaagc
      481 tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc aggtgatttc
      541 tgcatagcca gacttggggg tgatgagttt accttcaaga aactgatcag ggatagcggt
      601 caggtgtttt tacaaccact aaacccacag tacccaatga tcccatgcaa tgagagttgt
      661 tccgttgtgg ggaaagttat cgctagtcag tggcctgaag agacgtttgg cgctgcaaac
      721 gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta
      781 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      841 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      901 tctgcgttta tatactagag tccctatcag tgatagagat tgacatccct atcagtgata
      961 gagatactga gcactactag agaaagagga gaaatactag atgcgtaaag gagaagaact
     1021 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
     1081 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
     1141 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg
     1201 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc
     1261 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
     1321 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
     1381 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
     1441 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
     1501 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
     1561 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
     1621 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
     1681 tgggattaca catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa
     1741 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1801 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//