LOCUS       BBa_S03808              1629 bp    DNA     linear       21-May-2024
DEFINITION  cis3-repressed, tet-regulated Q
ACCESSION   BBa_S03808
VERSION     BBa_S03808.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=pTet
     misc_feature    55..106
                     /label=cis3 + RBS
     misc_feature    107..738
                     /label=Q
     misc_feature    1..738
                     /label=BBa_I759011
     promoter        747..781
                     /label=BBa_J23116
     misc_feature    794..797
                     /label=conserved
     RBS             790..801
                     /label=BBa_B0034
     CDS             811..1427
                     /label=tetR
     misc_feature    1428..1461
                     /label=SsrA
     misc_feature    1468..1492
                     /label=Help:Barcodes
     CDS             808..1467
                     /label=BBa_C0040
     stem_loop       1512..1555
                     /label=stem_loop
     misc_feature    1501..1580
                     /label=BBa_B0010
     terminator      1501..1580
                     /label=BBa_B0010
     stem_loop       1596..1615
                     /label=T7 TE
     misc_feature    1622..1622
                     /label=stop
     polyA_site      1616..1629
                     /label=polya
     misc_feature    1589..1629
                     /label=BBa_B0012
     terminator      1589..1629
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgtgcac
       61 attgattcac ctcttggagt tgggtattaa agaggagaaa caattgatga gactcgaaag
      121 cgtagctaaa tttcattcgc caaaaagccc gatgatgagc gactcaccac gggccacggc
      181 ttctgactct ctttccggta ctgatgtgat ggctgctatg gggatggcgc aatcacaagc
      241 cggattcggt atggctgcat tctgcggtaa gcacgaactc agccagaacg acaaacaaaa
      301 ggctatcaac tatctgatgc aatttgcaca caaggtatcg gggaaatacc gtggtgtggc
      361 aaagcttgaa ggaaatacta aggcaaaggt actgcaagtg ctcgcaacat tcgcttatgc
      421 ggattattgc cgtagtgccg cgacgccggg ggcaagatgc agagattgcc atggtacagg
      481 ccgtgcggtt gatattgcca aaacagagct gtgggggaga gttgtcgaga aagagtgcgg
      541 aagatgcaaa ggcgtcggct attcaaggat gccagcaagc gcagcatatc gcgctgtgac
      601 gatgctaatc ccaaacctta cccaacccac ctggtcacgc actgttaagc cgctgtatga
      661 cgctctggtg gtgcaatgcc acaaagaaga gtcaatcgca gacaacattt tgaatgcggt
      721 cacacgttag tactagagta ctagagttga cagctagctc agtcctaggg actatgctag
      781 ctactagaga aagaggagaa atactagatg tccagattag ataaaagtaa agtgattaac
      841 agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc
      901 cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg
      961 ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa
     1021 ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta
     1081 agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa
     1141 actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta
     1201 tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat
     1261 caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga
     1321 caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa
     1381 ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac
     1441 gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag
     1501 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1561 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1621 gcgtttata
//