LOCUS       BBa_S03816              2580 bp    DNA     linear       22-Dec-2024
DEFINITION  C0261:S03814
ACCESSION   BBa_S03816
VERSION     BBa_S03816.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    598..629
                     /label=LVA
     CDS             19..597
                     /label=luxI
     misc_feature    19..636
                     /label=BBa_C0061
     misc_feature    637..661
                     /label=Help:Barcodes
     CDS             19..636
                     /label=BBa_C0061
     misc_feature    1..661
                     /label=BBa_C0261
     misc_feature    674..677
                     /label=conserved
     RBS             670..681
                     /label=BBa_B0034
     misc_feature    1777..1815
                     /label=LVA
     CDS             688..1815
                     /label=lacI-LVA
     misc_feature    688..1815
                     /label=BBa_C0012
     misc_feature    1816..1840
                     /label=Help:Barcodes
     CDS             688..1815
                     /label=BBa_C0012
     misc_feature    670..1840
                     /label=BBa_S0100
     misc_feature    1853..1856
                     /label=conserved
     RBS             1849..1860
                     /label=BBa_B0034
     CDS             1867..2577
                     /label=mCherry
     misc_feature    2218..2218
                     /label=C->T (removing PstI site)
     misc_feature    1867..2580
                     /label=BBa_J06504
     misc_feature    1849..2580
                     /label=BBa_J06602
     misc_feature    670..2580
                     /label=BBa_S03814
ORIGIN
        1 aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca
       61 tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag
      121 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa
      181 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca
      241 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc
      301 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata
      361 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt
      421 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag
      481 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact
      541 aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct
      601 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct
      661 ctactagaga aagaggagaa atactagatg gtgaatgtga aaccagtaac gttatacgat
      721 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc
      781 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt
      841 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc
      901 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat
      961 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa
     1021 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg
     1081 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt
     1141 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga
     1201 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca
     1261 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat
     1321 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa
     1381 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag
     1441 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc
     1501 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc
     1561 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct
     1621 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc
     1681 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1741 ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga aaactacgct
     1801 ttagtagctt aataactctg atagtgctag tgtagatctc tactagagaa agaggagaaa
     1861 tactagatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     1921 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     1981 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     2041 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     2101 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     2161 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccttg
     2221 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     2281 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     2341 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     2401 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     2461 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     2521 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaataa
//