##gff-version 3
##sequence-region BBa_S03816 1 2580
BBa_S03816	.	engineered_region	1	661	.	+	0	ID=BBa_C0261;Name=BBa_C0261
BBa_S03816	.	CDS	19	636	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_S03816	.	engineered_region	19	636	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S03816	.	tag	598	629	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S03816	.	CDS	19	597	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S03816	.	engineered_tag	637	661	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S03816	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_S03816	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03816	.	engineered_region	670	2580	.	+	0	ID=BBa_S03814;Name=BBa_S03814
BBa_S03816	.	engineered_region	1849	2580	.	+	0	ID=BBa_J06602;Name=BBa_J06602;Parent=BBa_S03814
BBa_S03816	.	ribosome_entry_site	1849	1860	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06602
BBa_S03816	.	conserved_region	1853	1856	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03816	.	engineered_region	1867	2580	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06602
BBa_S03816	.	sequence_alteration	2218	2218	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_S03816	.	CDS	1867	2577	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_S03816	.	engineered_region	670	1840	.	+	0	ID=BBa_S0100;Name=BBa_S0100;Parent=BBa_S03814
BBa_S03816	.	ribosome_entry_site	670	681	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0100
BBa_S03816	.	conserved_region	674	677	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03816	.	CDS	688	1815	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S0100
BBa_S03816	.	engineered_tag	1816	1840	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S03816	.	CDS	688	1815	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S03816	.	engineered_region	688	1815	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S03816	.	tag	1777	1815	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
>BBa_S03816
aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggta
ttctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatga
gtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacct
acaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatag
tcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatt
tgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgattt
ttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtat
tgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacg
ttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctg
cgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcggg
caaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaa
tctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcgg
tgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtgga
agctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccat
gaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaa
gttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacg
ggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatg
ctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatct
cggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcct
gctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtc
tcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgc
agctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataact
ctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatg
gccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcg
agggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctg
ggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaag
ctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggact
cctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgca
gaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcag
aggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgc
ccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacg
cgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa