##gff-version 3
##sequence-region BBa_S03824 1 1876
BBa_S03824	.	engineered_region	1	1815	.	+	0	ID=BBa_S03823;Name=BBa_S03823
BBa_S03824	.	engineered_region	1	923	.	+	0	ID=BBa_I13521;Name=BBa_I13521;Parent=BBa_S03823
BBa_S03824	.	terminator	795	874	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13521
BBa_S03824	.	engineered_region	795	874	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03824	.	stem_loop	806	849	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03824	.	terminator	883	923	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13521
BBa_S03824	.	polyA_site	910	923	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03824	.	engineered_region	883	923	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03824	.	stem_loop	890	909	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03824	.	stop_codon	916	916	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03824	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13521
BBa_S03824	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03824	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13521
BBa_S03824	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S03824	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S03824	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S03824	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S03824	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S03824	.	CDS	81	761	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13521
BBa_S03824	.	engineered_tag	762	786	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_S03824	.	CDS	81	755	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S03824	.	engineered_region	932	1815	.	+	0	ID=BBa_I1466;Name=BBa_I1466;Parent=BBa_S03823
BBa_S03824	.	terminator	1775	1815	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I1466
BBa_S03824	.	polyA_site	1802	1815	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03824	.	engineered_region	1775	1815	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03824	.	stem_loop	1782	1801	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03824	.	stop_codon	1808	1808	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03824	.	terminator	1687	1766	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I1466
BBa_S03824	.	engineered_region	1687	1766	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03824	.	stem_loop	1698	1741	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03824	.	ribosome_entry_site	932	943	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I1466
BBa_S03824	.	conserved_region	936	939	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03824	.	CDS	950	1678	.	+	0	ID=BBa_C0171;Name=BBa_C0171;Parent=BBa_I1466
BBa_S03824	.	promoter	1824	1876	.	+	0	ID=BBa_R0071;Name=BBa_R0071
BBa_S03824	.	promoter	1865	1870	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_S03824	.	start_codon	1876	1876	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_S03824	.	non_covalent_binding_site	1824	1843	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_S03824	.	promoter	1842	1847	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
>BBa_S03824
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgag
ttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtc
cgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacat
cccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtt
accgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccg
acggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaa
aggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaa
aaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcg
ttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaaagaggagaaatacta
gatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggc
gtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattccct
tcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggc
cgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccgg
atgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctca
gcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcat
gatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgc
gaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagca
cggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggc
gctgggtctcatctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata
ctagagtcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttc