LOCUS BBa_S03828 5073 bp DNA linear 22-Dec-2024 DEFINITION P0412:I722008 ACCESSION BBa_S03828 VERSION BBa_S03828.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 1108..1146 /label=LVA CDS 19..1146 /label=lacI-LVA misc_feature 19..1146 /label=BBa_C0012 misc_feature 1147..1171 /label=Help:Barcodes CDS 19..1146 /label=BBa_C0012 stem_loop 1191..1234 /label=stem_loop misc_feature 1180..1259 /label=BBa_B0010 terminator 1180..1259 /label=BBa_B0010 stem_loop 1275..1294 /label=T7 TE misc_feature 1301..1301 /label=stop polyA_site 1295..1308 /label=polya misc_feature 1268..1308 /label=BBa_B0012 terminator 1268..1308 /label=BBa_B0012 misc_feature 1..1308 /label=BBa_P0412 CDS 1317..1404 /label=end of LacI coding region (inactive) misc_feature 1317..1516 /label=BBa_R0010 misc_feature 1405..1442 /label=CAP binding site promoter 1453..1458 /label=-35 promoter 1477..1482 /label=-10 misc_feature 1482..1516 /label=LacI binding site misc_feature 1489..1489 /label=start promoter 1317..1516 /label=BBa_R0010 misc_feature 1529..1532 /label=conserved RBS 1525..1536 /label=BBa_B0034 misc_feature 2122..2153 /label=LVA CDS 1543..2121 /label=luxI misc_feature 1543..2160 /label=BBa_C0061 misc_feature 2161..2185 /label=Help:Barcodes CDS 1543..2160 /label=BBa_C0061 misc_feature 1525..2185 /label=BBa_C0261 misc_feature 2198..2201 /label=conserved RBS 2194..2205 /label=BBa_B0034 CDS 2215..2922 /label=cI lambda misc_feature 2923..2955 /label=LVA misc_feature 2962..2986 /label=Help:Barcodes CDS 2212..2961 /label=BBa_C0051 stem_loop 3006..3049 /label=stem_loop misc_feature 2995..3074 /label=BBa_B0010 terminator 2995..3074 /label=BBa_B0010 stem_loop 3090..3109 /label=T7 TE misc_feature 3116..3116 /label=stop polyA_site 3110..3123 /label=polya misc_feature 3083..3123 /label=BBa_B0012 terminator 3083..3123 /label=BBa_B0012 promoter 3169..3174 /label=-10 promoter 3146..3151 /label=-35 misc_feature 3156..3172 /label=OR1 misc_feature 3132..3148 /label=OR2 misc_feature 3132..3180 /label=BBa_R0051 promoter 3132..3180 /label=BBa_R0051 misc_feature 2194..3180 /label=BBa_Q04510 misc_feature 1317..3180 /label=BBa_I722005 misc_feature 3193..3196 /label=conserved RBS 3189..3200 /label=BBa_B0034 misc_feature 3207..3208 /label=prefix misc_feature 3380..3380 /label=T misc_feature 3698..3698 /label=A CDS 3207..3956 /label=luxR misc_feature 3207..3962 /label=BBa_C0062 misc_feature 3963..3987 /label=Help:Barcodes CDS 3207..3962 /label=BBa_C0062 stem_loop 4007..4050 /label=stem_loop misc_feature 3996..4075 /label=BBa_B0010 terminator 3996..4075 /label=BBa_B0010 stem_loop 4091..4110 /label=T7 TE misc_feature 4117..4117 /label=stop polyA_site 4111..4124 /label=polya misc_feature 4084..4124 /label=BBa_B0012 terminator 4084..4124 /label=BBa_B0012 misc_feature 4133..4152 /label=LuxR/HSL promoter 4152..4157 /label=-35 promoter 4174..4179 /label=-10 misc_feature 4185..4185 /label=start misc_feature 4133..4187 /label=BBa_R0062 promoter 4133..4187 /label=BBa_R0062 misc_feature 3189..4187 /label=BBa_A340620 misc_feature 1317..4187 /label=BBa_S03827 RBS 4196..4210 /label=RBS-1\Strong RBS 4203..4207 /label=RBS misc_feature 4196..4210 /label=BBa_B0030 RBS 4196..4210 /label=BBa_B0030 CDS 4217..4936 /label=GFP protein CDS 4217..4936 /label=BBa_E0040 stem_loop 4956..4999 /label=stem_loop misc_feature 4945..5024 /label=BBa_B0010 terminator 4945..5024 /label=BBa_B0010 stem_loop 5040..5059 /label=T7 TE misc_feature 5066..5066 /label=stop polyA_site 5060..5073 /label=polya misc_feature 5033..5073 /label=BBa_B0012 terminator 5033..5073 /label=BBa_B0012 misc_feature 4196..5073 /label=BBa_E0840 misc_feature 1317..5073 /label=BBa_I722008 ORIGIN 1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag 61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct 121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc 181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg 241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt 301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg 361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag 421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct 481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg 541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct 601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag 661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa 721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg 781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg 841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag 901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag 961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg 1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct 1141 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa 1201 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 1261 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagcaat 1321 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 1381 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 1441 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 1501 ataacaattt cacacatact agagaaagag gagaaatact agatgactat aatgataaaa 1561 aaatcggatt ttttggcaat tccatcggag gagtataaag gtattctaag tcttcgttat 1621 caagtgttta agcaaagact tgagtgggac ttagttgtag aaaataacct tgaatcagat 1681 gagtatgata actcaaatgc agaatatatt tatgcttgtg atgatactga aaatgtaagt 1741 ggatgctggc gtttattacc tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa 1801 ttgcttggtc aacagagtgc tcccaaagat cctaatatag tcgaattaag tcgttttgct 1861 gtaggtaaaa atagctcaaa gataaataac tctgctagtg aaattacaat gaaactattt 1921 gaagctatat ataaacacgc tgttagtcaa ggtattacag aatatgtaac agtaacatca 1981 acagcaatag agcgattttt aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa 2041 gaaattcatg tattaggtga tactaaatcg gttgtattgt ctatgcctat taatgaacag 2101 tttaaaaaag cagtcttaaa tgctgcaaac gacgaaaact acgctttagt agcttaataa 2161 ctctgatagt gctagtgtag atctctacta gagaaagagg agaaatacta gatgagcaca 2221 aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa agcaatttat 2281 gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa gatggggatg 2341 gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc ttataacgcc 2401 gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc aatcgccaga 2461 gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag tgagtatgag 2521 taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag aacctttacc 2581 aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc tgcattctgg 2641 cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag ctttcctgac 2701 ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt ctgcatagcc 2761 agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg tcaggtgttt 2821 ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg ttccgttgtg 2881 gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gcgctgcaaa cgacgaaaac 2941 tacgctttag tagcttaata acgctgatag tgctagtgta gatcgctact agagccaggc 3001 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 3061 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 3121 atatactaga gtaacaccgt gcgtgttgac tattttacct ctggcggtga taatggttgc 3181 tactagagaa agaggagaaa tactagatga aaaacataaa tgccgacgac acatacagaa 3241 taattaataa aattaaagct tgtagaagca ataatgatat taatcaatgc ttatctgata 3301 tgactaaaat ggtacattgt gaatattatt tactcgcgat catttatcct cattctatgg 3361 ttaaatctga tatttcaatc ctagataatt accctaaaaa atggaggcaa tattatgatg 3421 acgctaattt aataaaatat gatcctatag tagattattc taactccaat cattcaccaa 3481 ttaattggaa tatatttgaa aacaatgctg taaataaaaa atctccaaat gtaattaaag 3541 aagcgaaaac atcaggtctt atcactgggt ttagtttccc tattcatacg gctaacaatg 3601 gcttcggaat gcttagtttt gcacattcag aaaaagacaa ctatatagat agtttatttt 3661 tacatgcgtg tatgaacata ccattaattg ttccttctct agttgataat tatcgaaaaa 3721 taaatatagc aaataataaa tcaaacaacg atttaaccaa aagagaaaaa gaatgtttag 3781 cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa aatattaggt tgcagtgagc 3841 gtactgtcac tttccattta accaatgcgc aaatgaaact caatacaaca aaccgctgcc 3901 aaagtatttc taaagcaatt ttaacaggag caattgattg cccatacttt aaaaattaat 3961 aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa aacgaaaggc 4021 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 4081 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agacctgtag 4141 gatcgtacag gtttacgcaa gaaaatggtt tgttatagtc gaataaatac tagagattaa 4201 agaggagaaa tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct 4261 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg 4321 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt 4381 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc 4441 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga 4501 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga 4561 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa 4621 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga 4681 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag 4741 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt 4801 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag 4861 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga 4921 actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 4981 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 5041 gctcaccttc gggtgggcct ttctgcgttt ata //