##gff-version 3
##sequence-region BBa_S03828 1 5073
BBa_S03828	.	engineered_region	1	1308	.	+	0	ID=BBa_P0412;Name=BBa_P0412
BBa_S03828	.	terminator	1268	1308	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0412
BBa_S03828	.	polyA_site	1295	1308	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03828	.	engineered_region	1268	1308	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03828	.	stem_loop	1275	1294	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03828	.	stop_codon	1301	1301	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03828	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0412
BBa_S03828	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03828	.	terminator	1180	1259	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0412
BBa_S03828	.	stem_loop	1191	1234	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03828	.	engineered_region	1180	1259	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03828	.	CDS	19	1146	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0412
BBa_S03828	.	engineered_region	19	1146	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S03828	.	engineered_tag	1147	1171	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S03828	.	tag	1108	1146	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S03828	.	CDS	19	1146	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S03828	.	engineered_region	1317	5073	.	+	0	ID=BBa_I722008;Name=BBa_I722008
BBa_S03828	.	engineered_region	4196	5073	.	+	0	ID=BBa_E0840;Name=BBa_E0840;Parent=BBa_I722008
BBa_S03828	.	terminator	5033	5073	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_S03828	.	polyA_site	5060	5073	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03828	.	engineered_region	5033	5073	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03828	.	stem_loop	5040	5059	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03828	.	stop_codon	5066	5066	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03828	.	ribosome_entry_site	4196	4210	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_S03828	.	ribosome_entry_site	4203	4207	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S03828	.	ribosome_entry_site	4196	4210	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S03828	.	engineered_region	4196	4210	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S03828	.	CDS	4217	4936	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_S03828	.	CDS	4217	4936	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_S03828	.	terminator	4945	5024	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_S03828	.	stem_loop	4956	4999	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03828	.	engineered_region	4945	5024	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03828	.	engineered_region	1317	4187	.	+	0	ID=BBa_S03827;Name=BBa_S03827;Parent=BBa_I722008
BBa_S03828	.	engineered_region	3189	4187	.	+	0	ID=BBa_A340620;Name=BBa_A340620;Parent=BBa_S03827
BBa_S03828	.	promoter	4133	4187	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_A340620
BBa_S03828	.	engineered_region	4133	4187	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_S03828	.	promoter	4152	4157	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_S03828	.	promoter	4174	4179	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_S03828	.	start_codon	4185	4185	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_S03828	.	non_covalent_binding_site	4133	4152	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_S03828	.	terminator	3996	4075	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_A340620
BBa_S03828	.	stem_loop	4007	4050	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03828	.	engineered_region	3996	4075	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03828	.	CDS	3207	3962	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_A340620
BBa_S03828	.	engineered_tag	3963	3987	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S03828	.	engineered_region	3207	3962	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S03828	.	CDS	3207	3956	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S03828	.	point_mutation	3380	3380	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S03828	.	point_mutation	3698	3698	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S03828	.	engineered_region	3207	3208	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S03828	.	terminator	4084	4124	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_A340620
BBa_S03828	.	polyA_site	4111	4124	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03828	.	engineered_region	4084	4124	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03828	.	stem_loop	4091	4110	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03828	.	stop_codon	4117	4117	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03828	.	ribosome_entry_site	3189	3200	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_A340620
BBa_S03828	.	conserved_region	3193	3196	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03828	.	engineered_region	1317	3180	.	+	0	ID=BBa_I722005;Name=BBa_I722005;Parent=BBa_S03827
BBa_S03828	.	promoter	1317	1516	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_I722005
BBa_S03828	.	start_codon	1489	1489	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S03828	.	CDS	1317	1404	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S03828	.	promoter	1477	1482	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S03828	.	non_covalent_binding_site	1482	1516	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S03828	.	non_covalent_binding_site	1405	1442	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S03828	.	engineered_region	1317	1516	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S03828	.	promoter	1453	1458	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S03828	.	engineered_region	2194	3180	.	+	0	ID=BBa_Q04510;Name=BBa_Q04510;Parent=BBa_I722005
BBa_S03828	.	terminator	3083	3123	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04510
BBa_S03828	.	polyA_site	3110	3123	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03828	.	engineered_region	3083	3123	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03828	.	stem_loop	3090	3109	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03828	.	stop_codon	3116	3116	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03828	.	CDS	2212	2961	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_Q04510
BBa_S03828	.	CDS	2215	2922	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_S03828	.	tag	2923	2955	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_S03828	.	engineered_tag	2962	2986	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_S03828	.	promoter	3132	3180	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_Q04510
BBa_S03828	.	non_covalent_binding_site	3156	3172	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_S03828	.	promoter	3146	3151	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_S03828	.	engineered_region	3132	3180	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_S03828	.	non_covalent_binding_site	3132	3148	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_S03828	.	promoter	3169	3174	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_S03828	.	terminator	2995	3074	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04510
BBa_S03828	.	stem_loop	3006	3049	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03828	.	engineered_region	2995	3074	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03828	.	ribosome_entry_site	2194	2205	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04510
BBa_S03828	.	conserved_region	2198	2201	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03828	.	engineered_region	1525	2185	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_I722005
BBa_S03828	.	CDS	1543	2160	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_S03828	.	engineered_region	1543	2160	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S03828	.	tag	2122	2153	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S03828	.	engineered_tag	2161	2185	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S03828	.	CDS	1543	2121	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S03828	.	ribosome_entry_site	1525	1536	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_S03828	.	conserved_region	1529	1532	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S03828
aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatc
agaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcgga
gctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagt
ctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgt
cgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgat
cattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgat
gtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcg
cattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggca
taaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaa
accatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgc
gcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatg
ttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactc
tctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaata
cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca
ggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgc
gttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgt
gagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgga
taacaatttcacacatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaat
tccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgta
gaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaa
gtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagag
tgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgct
agtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaa
catcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtatt
aggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgac
gaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactaga
tgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaa
aaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaat
ggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagccctt
caatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgt
tttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagc
acaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagc
caagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagact
tgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtac
ccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcg
ctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacct
ctggcggtgataatggttgctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacaga
ataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacatt
gtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaa
aaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattca
ccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcag
gtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaa
agacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattat
cgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcg
aaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgca
aatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatacttt
aaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
tactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttga
attagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacc
cttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaat
gctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaag
aactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaataga
atcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacaca
atgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaag
cgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctg
tccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctg
ggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttata