##gff-version 3
##sequence-region BBa_S03829 1 4249
BBa_S03829	.	engineered_region	1	1370	.	+	0	ID=BBa_I722007;Name=BBa_I722007
BBa_S03829	.	engineered_region	63	1370	.	+	0	ID=BBa_P0412;Name=BBa_P0412;Parent=BBa_I722007
BBa_S03829	.	terminator	1330	1370	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0412
BBa_S03829	.	polyA_site	1357	1370	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03829	.	engineered_region	1330	1370	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03829	.	stem_loop	1337	1356	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03829	.	stop_codon	1363	1363	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03829	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0412
BBa_S03829	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03829	.	terminator	1242	1321	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0412
BBa_S03829	.	stem_loop	1253	1296	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03829	.	engineered_region	1242	1321	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03829	.	CDS	81	1208	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0412
BBa_S03829	.	engineered_region	81	1208	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S03829	.	engineered_tag	1209	1233	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S03829	.	tag	1170	1208	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S03829	.	CDS	81	1208	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S03829	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I722007
BBa_S03829	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S03829	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S03829	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S03829	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S03829	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S03829	.	engineered_region	1379	4249	.	+	0	ID=BBa_S03827;Name=BBa_S03827
BBa_S03829	.	engineered_region	3251	4249	.	+	0	ID=BBa_A340620;Name=BBa_A340620;Parent=BBa_S03827
BBa_S03829	.	promoter	4195	4249	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_A340620
BBa_S03829	.	engineered_region	4195	4249	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_S03829	.	promoter	4214	4219	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_S03829	.	promoter	4236	4241	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_S03829	.	start_codon	4247	4247	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_S03829	.	non_covalent_binding_site	4195	4214	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_S03829	.	terminator	4058	4137	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_A340620
BBa_S03829	.	stem_loop	4069	4112	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03829	.	engineered_region	4058	4137	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03829	.	CDS	3269	4024	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_A340620
BBa_S03829	.	engineered_tag	4025	4049	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S03829	.	engineered_region	3269	4024	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S03829	.	CDS	3269	4018	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S03829	.	point_mutation	3442	3442	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S03829	.	point_mutation	3760	3760	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S03829	.	engineered_region	3269	3270	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S03829	.	terminator	4146	4186	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_A340620
BBa_S03829	.	polyA_site	4173	4186	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03829	.	engineered_region	4146	4186	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03829	.	stem_loop	4153	4172	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03829	.	stop_codon	4179	4179	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03829	.	ribosome_entry_site	3251	3262	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_A340620
BBa_S03829	.	conserved_region	3255	3258	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03829	.	engineered_region	1379	3242	.	+	0	ID=BBa_I722005;Name=BBa_I722005;Parent=BBa_S03827
BBa_S03829	.	promoter	1379	1578	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_I722005
BBa_S03829	.	start_codon	1551	1551	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S03829	.	CDS	1379	1466	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S03829	.	promoter	1539	1544	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S03829	.	non_covalent_binding_site	1544	1578	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S03829	.	non_covalent_binding_site	1467	1504	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S03829	.	engineered_region	1379	1578	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S03829	.	promoter	1515	1520	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S03829	.	engineered_region	2256	3242	.	+	0	ID=BBa_Q04510;Name=BBa_Q04510;Parent=BBa_I722005
BBa_S03829	.	terminator	3145	3185	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04510
BBa_S03829	.	polyA_site	3172	3185	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03829	.	engineered_region	3145	3185	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03829	.	stem_loop	3152	3171	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03829	.	stop_codon	3178	3178	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03829	.	CDS	2274	3023	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_Q04510
BBa_S03829	.	CDS	2277	2984	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_S03829	.	tag	2985	3017	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_S03829	.	engineered_tag	3024	3048	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_S03829	.	promoter	3194	3242	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_Q04510
BBa_S03829	.	non_covalent_binding_site	3218	3234	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_S03829	.	promoter	3208	3213	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_S03829	.	engineered_region	3194	3242	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_S03829	.	non_covalent_binding_site	3194	3210	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_S03829	.	promoter	3231	3236	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_S03829	.	terminator	3057	3136	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04510
BBa_S03829	.	stem_loop	3068	3111	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03829	.	engineered_region	3057	3136	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03829	.	ribosome_entry_site	2256	2267	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04510
BBa_S03829	.	conserved_region	2260	2263	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03829	.	engineered_region	1587	2247	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_I722005
BBa_S03829	.	CDS	1605	2222	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_S03829	.	engineered_region	1605	2222	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S03829	.	tag	2184	2215	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S03829	.	engineered_tag	2223	2247	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S03829	.	CDS	1605	2183	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S03829	.	ribosome_entry_site	1587	1598	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_S03829	.	conserved_region	1591	1594	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S03829
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtg
gtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattccca
accgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgcc
gtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagc
ggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctgg
atgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacc
catcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaa
atcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgca
atcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaa
tgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtcc
gggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaa
ccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggt
gaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccc
cgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaa
actacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaa
tgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcatt
aggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacata
ctagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtata
aaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatc
agatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgttta
ttacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatccta
atatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaa
actatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagag
cgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcgg
ttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagt
agcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgagcacaaaaaagaaa
ccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttat
cccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaa
tgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatc
tacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcagg
cagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccag
tgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacgga
atgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagttta
ccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaa
tgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaac
tacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggc
tcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacct
tcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggt
tgctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaa
agcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactc
gcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatatt
atgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatat
atttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggttt
agtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagata
gtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagc
aaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgg
gatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaa
caaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactg
atagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
actagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa