LOCUS BBa_S03840 2805 bp DNA linear 25-Nov-2024 DEFINITION S03839:Q04510 ACCESSION BBa_S03840 VERSION BBa_S03840.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 RBS 1070..1084 /label=RBS-1\Strong RBS 1077..1081 /label=RBS misc_feature 1070..1084 /label=BBa_B0030 RBS 1070..1084 /label=BBa_B0030 CDS 1091..1810 /label=GFP protein CDS 1091..1810 /label=BBa_E0040 misc_feature 1070..1810 /label=BBa_E5500 misc_feature 1..1810 /label=BBa_S03839 misc_feature 1823..1826 /label=conserved RBS 1819..1830 /label=BBa_B0034 CDS 1840..2547 /label=cI lambda misc_feature 2548..2580 /label=LVA misc_feature 2587..2611 /label=Help:Barcodes CDS 1837..2586 /label=BBa_C0051 stem_loop 2631..2674 /label=stem_loop misc_feature 2620..2699 /label=BBa_B0010 terminator 2620..2699 /label=BBa_B0010 stem_loop 2715..2734 /label=T7 TE misc_feature 2741..2741 /label=stop polyA_site 2735..2748 /label=polya misc_feature 2708..2748 /label=BBa_B0012 terminator 2708..2748 /label=BBa_B0012 promoter 2794..2799 /label=-10 promoter 2771..2776 /label=-35 misc_feature 2781..2797 /label=OR1 misc_feature 2757..2773 /label=OR2 misc_feature 2757..2805 /label=BBa_R0051 promoter 2757..2805 /label=BBa_R0051 misc_feature 1819..2805 /label=BBa_Q04510 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga ttaaagagga 1081 gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga 1141 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc 1201 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg 1261 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca 1321 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac 1381 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga 1441 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct 1501 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca 1561 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca 1621 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga 1681 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca 1741 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata 1801 caaataataa tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta 1861 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat 1921 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt 1981 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa 2041 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg 2101 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct 2161 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag 2221 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt 2281 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc 2341 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat 2401 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac 2461 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct 2521 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct 2581 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa 2641 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 2701 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtaac 2761 accgtgcgtg ttgactattt tacctctggc ggtgataatg gttgc //