LOCUS       BBa_S03871              1772 bp    DNA     linear       17-May-2024
DEFINITION  S03870:I0462
ACCESSION   BBa_S03871
VERSION     BBa_S03871.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..102
                     /label=BBa_J01010
     CDS             109..828
                     /label=GFP protein
     CDS             109..828
                     /label=BBa_E0040
     misc_feature    1..828
                     /label=BBa_S03870
     misc_feature    841..844
                     /label=conserved
     RBS             837..848
                     /label=BBa_B0034
     misc_feature    855..856
                     /label=prefix
     misc_feature    1028..1028
                     /label=T
     misc_feature    1346..1346
                     /label=A
     CDS             855..1604
                     /label=luxR
     misc_feature    855..1610
                     /label=BBa_C0062
     misc_feature    1611..1635
                     /label=Help:Barcodes
     CDS             855..1610
                     /label=BBa_C0062
     stem_loop       1655..1698
                     /label=stem_loop
     misc_feature    1644..1723
                     /label=BBa_B0010
     terminator      1644..1723
                     /label=BBa_B0010
     stem_loop       1739..1758
                     /label=T7 TE
     misc_feature    1765..1765
                     /label=stop
     polyA_site      1759..1772
                     /label=polya
     misc_feature    1732..1772
                     /label=BBa_B0012
     terminator      1732..1772
                     /label=BBa_B0012
     misc_feature    837..1772
                     /label=BBa_I0462
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaactagaa tcacctcttg gatttgggta ttaaagagga gatactagat gcgtaaagga
      121 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      181 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      241 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
      301 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
      361 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
      421 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
      481 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
      541 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
      601 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
      661 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
      721 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
      781 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagaaag
      841 aggagaaata ctagatgaaa aacataaatg ccgacgacac atacagaata attaataaaa
      901 ttaaagcttg tagaagcaat aatgatatta atcaatgctt atctgatatg actaaaatgg
      961 tacattgtga atattattta ctcgcgatca tttatcctca ttctatggtt aaatctgata
     1021 tttcaatcct agataattac cctaaaaaat ggaggcaata ttatgatgac gctaatttaa
     1081 taaaatatga tcctatagta gattattcta actccaatca ttcaccaatt aattggaata
     1141 tatttgaaaa caatgctgta aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat
     1201 caggtcttat cactgggttt agtttcccta ttcatacggc taacaatggc ttcggaatgc
     1261 ttagttttgc acattcagaa aaagacaact atatagatag tttattttta catgcgtgta
     1321 tgaacatacc attaattgtt ccttctctag ttgataatta tcgaaaaata aatatagcaa
     1381 ataataaatc aaacaacgat ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg
     1441 aaggaaaaag ctcttgggat atttcaaaaa tattaggttg cagtgagcgt actgtcactt
     1501 tccatttaac caatgcgcaa atgaaactca atacaacaaa ccgctgccaa agtatttcta
     1561 aagcaatttt aacaggagca attgattgcc catactttaa aaattaataa cactgatagt
     1621 gctagtgtag atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1681 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1741 ctcaccttcg ggtgggcctt tctgcgttta ta
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