BBa_R0184
1
BBa_R0184
T7 promoter (lacI repressible)
2007-04-25T11:00:00Z
2015-05-08T01:14:15Z
This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -16.
A mutant version of the T7lac promoter designed by Dubendorff and Studier. The promoter is based on the strong ??10 T7 promoter but incorporates an A->C mutation at -16. The LacI operator site is a partially symmetric 25bp sequence.
false
false
_11_
0
135
84
Not in stock
false
The -16 mutation was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
false
Bartholomew Canton
annotation1932678
1
BBa_R0183 fragment
range1932678
1
1
20
annotation1932679
1
lacI binding site
range1932679
1
20
44
annotation1932680
1
Center of binding site symmetry
range1932680
1
31
31
BBa_B0073
1
BBa_B0073
Specialized RBS
2006-05-01T11:00:00Z
2015-08-31T04:07:21Z
-- No description --
false
false
_11_6_
0
135
6
Not in stock
false
false
Bartholomew Canton
BBa_S03909
1
BBa_S03909
R0184:B0073
2007-11-29T12:00:00Z
2015-05-08T01:14:32Z
false
false
_84_
0
135
84
Not in stock
false
false
Bartholomew Canton
component2218467
1
BBa_B0073
component2218466
1
BBa_R0184
annotation2218466
1
BBa_R0184
range2218466
1
1
44
annotation2218467
1
BBa_B0073
range2218467
1
53
61
BBa_S03909_sequence
1
tcatacgactcactataggggaattgtgagcggataacaattcctactagagtcacaccac
BBa_R0184_sequence
1
tcatacgactcactataggggaattgtgagcggataacaattcc
BBa_B0073_sequence
1
tcacaccac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z