LOCUS BBa_S03912 1056 bp DNA linear 25-Nov-2024 DEFINITION Trp-RBS-GFP.lva-TT ACCESSION BBa_S03912 VERSION BBa_S03912.1 FEATURES Location/Qualifiers RBS 5..13 /label=Predicted RBS misc_feature 20..22 /label=start misc_feature 47..52 /label=tandem trp codons misc_feature 62..64 /label=stop CDS 20..64 /label=leader peptide misc_feature 127..134 /label=Poly T promoter 1..134 /label=BBa_I715052 misc_feature 147..150 /label=conserved RBS 143..154 /label=BBa_B0034 misc_feature 161..880 /label=BBa_E0040 misc_feature 875..913 /label=LVA misc_feature 914..919 /label=Double Stop misc_feature 161..163 /label=Start misc_feature 161..919 /label=BBa_J04031 stem_loop 939..982 /label=stem_loop misc_feature 928..1007 /label=BBa_B0010 terminator 928..1007 /label=BBa_B0010 stem_loop 1023..1042 /label=T7 TE misc_feature 1049..1049 /label=stop polyA_site 1043..1056 /label=polya misc_feature 1016..1056 /label=BBa_B0012 terminator 1016..1056 /label=BBa_B0012 ORIGIN 1 cgtaaaaagg gtatcgacaa tgaaagcaat tttcgtactg aaaggttggt ggcgcacttc 61 ctgaaacggg cagtgtattc accatgcgta aagcaatcag atacccagcc cgcctaatga 121 gcgggctttt tttttactag agaaagagga gaaatactag atgcgtaaag gagaagaact 181 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt 241 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat 301 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg 361 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc 421 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa 481 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg 541 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc 601 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat 661 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc 721 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct 781 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc 841 tgggattaca catggcatgg atgaactata caaaaggcct gctgcaaacg acgaaaacta 901 cgctttagta gcttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 961 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 1021 ctggctcacc ttcgggtggg cctttctgcg tttata //