LOCUS       BBa_S03921              1017 bp    DNA     linear       22-Nov-2024
DEFINITION  Trp-RBS-GFP-TT
ACCESSION   BBa_S03921
VERSION     BBa_S03921.1  
FEATURES             Location/Qualifiers
     RBS             5..13
                     /label=Predicted RBS
     misc_feature    20..22
                     /label=start
     misc_feature    47..52
                     /label=tandem trp codons
     misc_feature    62..64
                     /label=stop
     CDS             20..64
                     /label=leader peptide
     misc_feature    127..134
                     /label=Poly T
     promoter        1..134
                     /label=BBa_I715052
     misc_feature    147..150
                     /label=conserved
     RBS             143..154
                     /label=BBa_B0034
     CDS             161..880
                     /label=GFP protein
     CDS             161..880
                     /label=BBa_E0040
     stem_loop       900..943
                     /label=stem_loop
     misc_feature    889..968
                     /label=BBa_B0010
     terminator      889..968
                     /label=BBa_B0010
     stem_loop       984..1003
                     /label=T7 TE
     misc_feature    1010..1010
                     /label=stop
     polyA_site      1004..1017
                     /label=polya
     misc_feature    977..1017
                     /label=BBa_B0012
     terminator      977..1017
                     /label=BBa_B0012
ORIGIN
        1 cgtaaaaagg gtatcgacaa tgaaagcaat tttcgtactg aaaggttggt ggcgcacttc
       61 ctgaaacggg cagtgtattc accatgcgta aagcaatcag atacccagcc cgcctaatga
      121 gcgggctttt tttttactag agaaagagga gaaatactag atgcgtaaag gagaagaact
      181 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      241 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
      301 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg
      361 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc
      421 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
      481 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
      541 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
      601 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
      661 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
      721 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
      781 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
      841 tgggattaca catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa
      901 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      961 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//