LOCUS       BBa_S03922              1042 bp    DNA     linear       29-Dec-2025
DEFINITION  I715053:I13504
ACCESSION   BBa_S03922
VERSION     BBa_S03922.1  
FEATURES             Location/Qualifiers
     misc_feature    20..22
                     /label=start
     RBS             5..13
                     /label=predicted RBS
     CDS             20..64
                     /label=leader peptide
     misc_feature    47..52
                     /label=tandem trp codons
     misc_feature    62..64
                     /label=stop
     misc_feature    152..159
                     /label=Poly T
     misc_feature    91..116
                     /label=hixC
     promoter        1..159
                     /label=BBa_I715053
     misc_feature    172..175
                     /label=conserved
     RBS             168..179
                     /label=BBa_B0034
     CDS             186..905
                     /label=GFP protein
     CDS             186..905
                     /label=BBa_E0040
     stem_loop       925..968
                     /label=stem_loop
     misc_feature    914..993
                     /label=BBa_B0010
     terminator      914..993
                     /label=BBa_B0010
     stem_loop       1009..1028
                     /label=T7 TE
     misc_feature    1035..1035
                     /label=stop
     polyA_site      1029..1042
                     /label=polya
     misc_feature    1002..1042
                     /label=BBa_B0012
     terminator      1002..1042
                     /label=BBa_B0012
ORIGIN
        1 cgtaaaaagg gtatcgacaa tgaaagcaat tttcgtactg aaaggttggt ggcgcacttc
       61 ctgaaacggg cagtgtattc accatgcgta ttatcaaaaa ccatggtttt tgataaagca
      121 atcagatacc cagcccgcct aatgagcggg cttttttttt actagagaaa gaggagaaat
      181 actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
      241 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
      301 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa
      361 cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga
      421 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
      481 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc
      541 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
      601 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga
      661 atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc gttcaactag
      721 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
      781 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
      841 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat
      901 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      961 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     1021 ggtgggcctt tctgcgttta ta
//