LOCUS BBa_S03940 3208 bp DNA linear 10-Jun-2024 DEFINITION J70009:pSB1AK3 ACCESSION BBa_S03940 VERSION BBa_S03940.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=PpiI misc_feature 9..11 /label=PpiI misc_feature 2..8 /label=MabI misc_feature 1..11 /label=BBa_J70009 misc_feature 20..40 /label=Suffix misc_feature 3187..3208 /label=Prefix CDS 280..894 /label=rep (pMB1) (rev) CDS 1116..1931 /label=kanR (rev) CDS 2086..2946 /label=ampR (rev) misc_feature 295..295 /label=ORI stem_loop 3158..3177 /label=terminator stem loop stem_loop 49..83 /label=terminator stem loop primer_bind 3069..3088 /label=VF2 primer_bind 176..195 /label=VR misc_feature 53..54 /label=deletion of two bases misc_feature 41..112 /label=E. coli his terminator misc_feature 3145..3148 /label=end of terminator misc_feature 20..3208 /label=pSB1AK3 ORIGIN 1 aaccaggtct ctactagagt actagtagcg gccgctgcag tccggcaaaa aaacgggcaa 61 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc 121 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 181 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 241 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 301 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 361 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 421 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 481 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 541 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 601 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 661 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 721 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 781 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 841 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 901 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 961 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 1021 ctaaagtata tatgagtaaa cttggtctga cagctcgagt cccgtcaagt cagcgtaatg 1081 ctctgccagt gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa 1141 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc 1201 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg 1261 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata 1321 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc 1381 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca 1441 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga 1501 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc 1561 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt 1621 ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg 1681 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca 1741 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca 1801 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca 1861 tataaatcag catccatgtt ggaatttaat cgcggcctgg agcaagacgt ttcccgttga 1921 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat 1981 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt 2041 tgttgaataa atcgaacttt tgctgagttg aaggatcagc tcgagttacc aatgcttaat 2101 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 2161 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 2221 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 2281 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 2341 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 2401 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 2461 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 2521 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 2581 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 2641 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 2701 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 2761 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 2821 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 2881 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 2941 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 3001 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 3061 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 3121 taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga 3181 tttctggaat tcgcggccgc ttctagag //