##gff-version 3
##sequence-region BBa_S03940 1 3208
BBa_S03940	.	plasmid_vector	20	3208	.	+	0	ID=pSB1AK3;Name=pSB1AK3
BBa_S03940	.	CDS	280	894	.	+	0	ID=annotation1780429;Name=rep (pMB1) (rev);Parent=pSB1AK3
BBa_S03940	.	engineered_region	20	40	.	+	0	ID=annotation1780400;Name=Suffix;Parent=pSB1AK3
BBa_S03940	.	sequence_feature	53	54	.	+	0	ID=annotation1823805;Name=deletion of two bases;Parent=pSB1AK3
BBa_S03940	.	primer_binding_site	176	195	.	+	0	ID=annotation1780436;Name=VR;Parent=pSB1AK3
BBa_S03940	.	primer_binding_site	3069	3088	.	+	0	ID=annotation1780435;Name=VF2;Parent=pSB1AK3
BBa_S03940	.	engineered_region	3187	3208	.	+	0	ID=annotation1780401;Name=Prefix;Parent=pSB1AK3
BBa_S03940	.	stem_loop	3158	3177	.	+	0	ID=annotation1780433;Name=terminator stem loop;Parent=pSB1AK3
BBa_S03940	.	CDS	1116	1931	.	+	0	ID=annotation1780430;Name=kanR (rev);Parent=pSB1AK3
BBa_S03940	.	CDS	2086	2946	.	+	0	ID=annotation1780431;Name=ampR (rev);Parent=pSB1AK3
BBa_S03940	.	stem_loop	49	83	.	+	0	ID=annotation1780434;Name=terminator stem loop;Parent=pSB1AK3
BBa_S03940	.	sequence_feature	3145	3148	.	+	0	ID=annotation1823993;Name=end of terminator;Parent=pSB1AK3
BBa_S03940	.	sequence_feature	295	295	.	+	0	ID=annotation1780432;Name=ORI;Parent=pSB1AK3
BBa_S03940	.	sequence_feature	41	112	.	+	0	ID=annotation1823992;Name=E. coli his terminator;Parent=pSB1AK3
BBa_S03940	.	sequence_feature	1	11	.	+	0	ID=BBa_J70009;Name=BBa_J70009
BBa_S03940	.	sequence_feature	9	11	.	+	0	ID=annotation1963024;Name=PpiI;Parent=BBa_J70009
BBa_S03940	.	sequence_feature	1	3	.	+	0	ID=annotation1963023;Name=PpiI ;Parent=BBa_J70009
BBa_S03940	.	sequence_feature	2	8	.	+	0	ID=annotation1963025;Name=MabI;Parent=BBa_J70009
>BBa_S03940
aaccaggtctctactagagtactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccct
ttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggc
tgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacat
gtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccct
gacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttcccc
ctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaag
cgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcac
gaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttat
cgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtg
gcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagtt
ggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaa
aaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggat
tttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagt
atatatgagtaaacttggtctgacagctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaac
caattctgattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttt
tgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctg
cgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcacc
atgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccatta
cgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgat
cgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttc
acctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatca
ggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaa
catcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgt
cgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggc
ctggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattg
ttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcg
aacttttgctgagttgaaggatcagctcgagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatt
tcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgct
gcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgca
gaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagt
taatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagc
tccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccga
tcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgcc
atccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgc
tcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttctt
cggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttc
agcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcg
acacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcg
gatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgt
ctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatcc
ttagctttcgctaaggatgatttctggaattcgcggccgcttctagag