LOCUS BBa_S03942 3206 bp DNA linear 25-Nov-2024 DEFINITION J70011:pSB1AK3 ACCESSION BBa_S03942 VERSION BBa_S03942.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=PsrI misc_feature 2..7 /label=MabI misc_feature 1..9 /label=BBa_J70011 misc_feature 18..38 /label=Suffix misc_feature 3185..3206 /label=Prefix CDS 278..892 /label=rep (pMB1) (rev) CDS 1114..1929 /label=kanR (rev) CDS 2084..2944 /label=ampR (rev) misc_feature 293..293 /label=ORI stem_loop 3156..3175 /label=terminator stem loop stem_loop 47..81 /label=terminator stem loop primer_bind 3067..3086 /label=VF2 primer_bind 174..193 /label=VR misc_feature 51..52 /label=deletion of two bases misc_feature 39..110 /label=E. coli his terminator misc_feature 3143..3146 /label=end of terminator misc_feature 18..3206 /label=pSB1AK3 ORIGIN 1 aaccaggtat actagagtac tagtagcggc cgctgcagtc cggcaaaaaa acgggcaagg 61 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt 121 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 181 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 241 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 301 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 361 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 421 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 481 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 541 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 601 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 661 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 721 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 781 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 841 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 901 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 961 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 1021 aaagtatata tgagtaaact tggtctgaca gctcgagtcc cgtcaagtca gcgtaatgct 1081 ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg 1141 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg 1201 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc 1261 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag 1321 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt 1381 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact 1441 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc 1501 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag 1561 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt 1621 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat 1681 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc 1741 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata 1801 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata 1861 taaatcagca tccatgttgg aatttaatcg cggcctggag caagacgttt cccgttgaat 1921 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga 1981 tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttg 2041 ttgaataaat cgaacttttg ctgagttgaa ggatcagctc gagttaccaa tgcttaatca 2101 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 2161 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 2221 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 2281 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 2341 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 2401 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 2461 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 2521 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 2581 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 2641 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 2701 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 2761 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 2821 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 2881 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 2941 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 3001 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 3061 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 3121 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt 3181 tctggaattc gcggccgctt ctagag //