##gff-version 3
##sequence-region BBa_S03942 1 3206
BBa_S03942	.	plasmid_vector	18	3206	.	+	0	ID=pSB1AK3;Name=pSB1AK3
BBa_S03942	.	stem_loop	3156	3175	.	+	0	ID=annotation1780433;Name=terminator stem loop;Parent=pSB1AK3
BBa_S03942	.	sequence_feature	51	52	.	+	0	ID=annotation1823805;Name=deletion of two bases;Parent=pSB1AK3
BBa_S03942	.	CDS	1114	1929	.	+	0	ID=annotation1780430;Name=kanR (rev);Parent=pSB1AK3
BBa_S03942	.	primer_binding_site	174	193	.	+	0	ID=annotation1780436;Name=VR;Parent=pSB1AK3
BBa_S03942	.	sequence_feature	3143	3146	.	+	0	ID=annotation1823993;Name=end of terminator;Parent=pSB1AK3
BBa_S03942	.	engineered_region	18	38	.	+	0	ID=annotation1780400;Name=Suffix;Parent=pSB1AK3
BBa_S03942	.	CDS	278	892	.	+	0	ID=annotation1780429;Name=rep (pMB1) (rev);Parent=pSB1AK3
BBa_S03942	.	engineered_region	3185	3206	.	+	0	ID=annotation1780401;Name=Prefix;Parent=pSB1AK3
BBa_S03942	.	sequence_feature	39	110	.	+	0	ID=annotation1823992;Name=E. coli his terminator;Parent=pSB1AK3
BBa_S03942	.	CDS	2084	2944	.	+	0	ID=annotation1780431;Name=ampR (rev);Parent=pSB1AK3
BBa_S03942	.	stem_loop	47	81	.	+	0	ID=annotation1780434;Name=terminator stem loop;Parent=pSB1AK3
BBa_S03942	.	primer_binding_site	3067	3086	.	+	0	ID=annotation1780435;Name=VF2;Parent=pSB1AK3
BBa_S03942	.	sequence_feature	293	293	.	+	0	ID=annotation1780432;Name=ORI;Parent=pSB1AK3
BBa_S03942	.	sequence_feature	1	9	.	+	0	ID=BBa_J70011;Name=BBa_J70011
BBa_S03942	.	sequence_feature	1	3	.	+	0	ID=annotation1963067;Name=PsrI;Parent=BBa_J70011
BBa_S03942	.	sequence_feature	2	7	.	+	0	ID=annotation1963068;Name=MabI;Parent=BBa_J70011
>BBa_S03942
aaccaggtatactagagtactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgcccttt
ttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctg
cggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgt
gagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctga
cgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccct
ggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcg
tggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacga
accccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcg
ccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggc
ctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttgg
tagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaa
aaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattt
tggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtat
atatgagtaaacttggtctgacagctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaacca
attctgattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttg
aaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcg
attccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccat
gagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacg
ctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcg
ctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcac
ctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcagg
agtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaaca
tcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcg
cacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcct
ggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgtt
catgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaa
cttttgctgagttgaaggatcagctcgagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttc
gttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgc
aatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcaga
agtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagtta
atagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctc
cggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatc
gttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccat
ccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctc
ttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcg
gggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcag
catcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgac
acggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcgga
tacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtct
aagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatcctt
agctttcgctaaggatgatttctggaattcgcggccgcttctagag