LOCUS BBa_S03943 3249 bp DNA linear 22-Nov-2024 DEFINITION J70012:pSB1AK3 ACCESSION BBa_S03943 VERSION BBa_S03943.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=PsrI 1 misc_feature 10..12 /label=PsrI 1 misc_feature 43..46 /label=PsrI 2 misc_feature 4..10 /label=Mab 1 misc_feature 47..52 /label=Mab 2 misc_feature 20..27 /label=AscI 1 misc_feature 28..34 /label=AscI 2 misc_feature 1..52 /label=BBa_J70012 misc_feature 61..81 /label=Suffix misc_feature 3228..3249 /label=Prefix CDS 321..935 /label=rep (pMB1) (rev) CDS 1157..1972 /label=kanR (rev) CDS 2127..2987 /label=ampR (rev) misc_feature 336..336 /label=ORI stem_loop 3199..3218 /label=terminator stem loop stem_loop 90..124 /label=terminator stem loop primer_bind 3110..3129 /label=VF2 primer_bind 217..236 /label=VR misc_feature 94..95 /label=deletion of two bases misc_feature 82..153 /label=E. coli his terminator misc_feature 3186..3189 /label=end of terminator misc_feature 61..3249 /label=pSB1AK3 ORIGIN 1 aacacctggt acagcaggtg gcgcgccggc gcgccagctg gtgaacacca ggtactagag 61 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 121 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 181 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 241 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 301 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 361 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 421 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 481 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 541 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 601 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 661 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 721 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 781 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 841 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 901 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 961 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 1021 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 1081 acttggtctg acagctcgag tcccgtcaag tcagcgtaat gctctgccag tgttacaacc 1141 aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca 1201 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact 1261 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc 1321 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat 1381 caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga 1441 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt 1501 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat 1561 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt 1621 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg 1681 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa 1741 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt 1801 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg 1861 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt 1921 tggaatttaa tcgcggcctg gagcaagacg tttcccgttg aatatggctc ataacacccc 1981 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt 2041 gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata aatcgaactt 2101 ttgctgagtt gaaggatcag ctcgagttac caatgcttaa tcagtgaggc acctatctca 2161 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 2221 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 2281 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 2341 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 2401 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 2461 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 2521 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 2581 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 2641 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 2701 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 2761 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 2821 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 2881 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 2941 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 3001 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 3061 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 3121 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag 3181 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg 3241 cttctagag //