##gff-version 3
##sequence-region BBa_S03943 1 3249
BBa_S03943	.	plasmid_vector	61	3249	.	+	0	ID=pSB1AK3;Name=pSB1AK3
BBa_S03943	.	engineered_region	3228	3249	.	+	0	ID=annotation1780401;Name=Prefix;Parent=pSB1AK3
BBa_S03943	.	sequence_feature	82	153	.	+	0	ID=annotation1823992;Name=E. coli his terminator;Parent=pSB1AK3
BBa_S03943	.	CDS	2127	2987	.	+	0	ID=annotation1780431;Name=ampR (rev);Parent=pSB1AK3
BBa_S03943	.	CDS	1157	1972	.	+	0	ID=annotation1780430;Name=kanR (rev);Parent=pSB1AK3
BBa_S03943	.	stem_loop	90	124	.	+	0	ID=annotation1780434;Name=terminator stem loop;Parent=pSB1AK3
BBa_S03943	.	CDS	321	935	.	+	0	ID=annotation1780429;Name=rep (pMB1) (rev);Parent=pSB1AK3
BBa_S03943	.	sequence_feature	336	336	.	+	0	ID=annotation1780432;Name=ORI;Parent=pSB1AK3
BBa_S03943	.	primer_binding_site	3110	3129	.	+	0	ID=annotation1780435;Name=VF2;Parent=pSB1AK3
BBa_S03943	.	stem_loop	3199	3218	.	+	0	ID=annotation1780433;Name=terminator stem loop;Parent=pSB1AK3
BBa_S03943	.	sequence_feature	94	95	.	+	0	ID=annotation1823805;Name=deletion of two bases;Parent=pSB1AK3
BBa_S03943	.	primer_binding_site	217	236	.	+	0	ID=annotation1780436;Name=VR;Parent=pSB1AK3
BBa_S03943	.	sequence_feature	3186	3189	.	+	0	ID=annotation1823993;Name=end of terminator;Parent=pSB1AK3
BBa_S03943	.	engineered_region	61	81	.	+	0	ID=annotation1780400;Name=Suffix;Parent=pSB1AK3
BBa_S03943	.	sequence_feature	1	52	.	+	0	ID=BBa_J70012;Name=BBa_J70012
BBa_S03943	.	sequence_feature	47	52	.	+	0	ID=annotation1963020;Name=Mab 2;Parent=BBa_J70012
BBa_S03943	.	sequence_feature	1	3	.	+	0	ID=annotation1963015;Name=PsrI 1;Parent=BBa_J70012
BBa_S03943	.	sequence_feature	4	10	.	+	0	ID=annotation1963018;Name=Mab 1;Parent=BBa_J70012
BBa_S03943	.	sequence_feature	10	12	.	+	0	ID=annotation1963016;Name=PsrI 1;Parent=BBa_J70012
BBa_S03943	.	non_covalent_binding_site	20	27	.	+	0	ID=annotation1963021;Name=AscI 1;Parent=BBa_J70012
BBa_S03943	.	sequence_feature	43	46	.	+	0	ID=annotation1963017;Name=PsrI 2;Parent=BBa_J70012
BBa_S03943	.	non_covalent_binding_site	28	34	.	+	0	ID=annotation1963022;Name=AscI 2 ;Parent=BBa_J70012
>BBa_S03943
aacacctggtacagcaggtggcgcgccggcgcgccagctggtgaacaccaggtactagagtactagtagcggccgctgc
agtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgca
ggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaata
cggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaa
aggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtg
gcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctg
ccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctca
gttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccgg
taactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcaga
gcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggta
tctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtag
cggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacg
gggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctaga
tccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgagtcccgt
caagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaaaaactcatcgagcatcaaatgaa
actgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcacc
gaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaat
ttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagct
tatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgtt
attcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgc
aaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctg
ttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcat
aaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaac
aactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttat
acccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgttgaatatggctcataac
accccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacat
cagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagctcgagttaccaa
tgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtaga
taactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccaga
tttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtct
attaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggca
tcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccc
catgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactc
atggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaa
ccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccaca
tagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgaga
tccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaa
aaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttca
atattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaata
ggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaa
ataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggcc
gcttctagag