LOCUS BBa_S03944 3250 bp DNA linear 01-Jun-2024 DEFINITION J70010:pSB1AK3 ACCESSION BBa_S03944 VERSION BBa_S03944.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=PpiI 1 misc_feature 9..12 /label=PpiI 1 misc_feature 42..45 /label=PpiI 2 misc_feature 51..53 /label=PpiI2 misc_feature 13..19 /label=MabI 1 misc_feature 35..41 /label=MabI 2 misc_feature 19..24 /label=PacI 1 misc_feature 27..34 /label=PacI 2 misc_feature 23..30 /label=PacI3 misc_feature 1..53 /label=BBa_J70010 misc_feature 62..82 /label=Suffix misc_feature 3229..3250 /label=Prefix CDS 322..936 /label=rep (pMB1) (rev) CDS 1158..1973 /label=kanR (rev) CDS 2128..2988 /label=ampR (rev) misc_feature 337..337 /label=ORI stem_loop 3200..3219 /label=terminator stem loop stem_loop 91..125 /label=terminator stem loop primer_bind 3111..3130 /label=VF2 primer_bind 218..237 /label=VR misc_feature 95..96 /label=deletion of two bases misc_feature 83..154 /label=E. coli his terminator misc_feature 3187..3190 /label=end of terminator misc_feature 62..3250 /label=pSB1AK3 ORIGIN 1 gagagctggt tcaccaggtt aattaattaa ttaaacctgg tgaacgtggt ctctactaga 61 gtactagtag cggccgctgc agtccggcaa aaaaacgggc aaggtgtcac caccctgccc 121 tttttcttta aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc tcactgactc 181 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 241 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 301 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 361 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 421 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 481 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 541 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 601 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 661 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 721 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 781 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 841 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 901 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 961 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 1021 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 1081 aacttggtct gacagctcga gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac 1141 caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc 1201 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac 1261 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt 1321 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa 1381 tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag 1441 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg 1501 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa 1561 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt 1621 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg 1681 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata 1741 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct 1801 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc 1861 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg 1921 ttggaattta atcgcggcct ggagcaagac gtttcccgtt gaatatggct cataacaccc 1981 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct 2041 tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact 2101 tttgctgagt tgaaggatca gctcgagtta ccaatgctta atcagtgagg cacctatctc 2161 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 2221 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 2281 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 2341 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 2401 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 2461 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 2521 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 2581 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 2641 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 2701 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 2761 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 2821 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 2881 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 2941 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 3001 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 3061 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 3121 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca 3181 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc 3241 gcttctagag //