##gff-version 3
##sequence-region BBa_S03944 1 3250
BBa_S03944	.	plasmid_vector	62	3250	.	+	0	ID=pSB1AK3;Name=pSB1AK3
BBa_S03944	.	CDS	2128	2988	.	+	0	ID=annotation1780431;Name=ampR (rev);Parent=pSB1AK3
BBa_S03944	.	engineered_region	62	82	.	+	0	ID=annotation1780400;Name=Suffix;Parent=pSB1AK3
BBa_S03944	.	sequence_feature	83	154	.	+	0	ID=annotation1823992;Name=E. coli his terminator;Parent=pSB1AK3
BBa_S03944	.	CDS	322	936	.	+	0	ID=annotation1780429;Name=rep (pMB1) (rev);Parent=pSB1AK3
BBa_S03944	.	primer_binding_site	3111	3130	.	+	0	ID=annotation1780435;Name=VF2;Parent=pSB1AK3
BBa_S03944	.	sequence_feature	3187	3190	.	+	0	ID=annotation1823993;Name=end of terminator;Parent=pSB1AK3
BBa_S03944	.	stem_loop	91	125	.	+	0	ID=annotation1780434;Name=terminator stem loop;Parent=pSB1AK3
BBa_S03944	.	sequence_feature	337	337	.	+	0	ID=annotation1780432;Name=ORI;Parent=pSB1AK3
BBa_S03944	.	sequence_feature	95	96	.	+	0	ID=annotation1823805;Name=deletion of two bases;Parent=pSB1AK3
BBa_S03944	.	stem_loop	3200	3219	.	+	0	ID=annotation1780433;Name=terminator stem loop;Parent=pSB1AK3
BBa_S03944	.	primer_binding_site	218	237	.	+	0	ID=annotation1780436;Name=VR;Parent=pSB1AK3
BBa_S03944	.	engineered_region	3229	3250	.	+	0	ID=annotation1780401;Name=Prefix;Parent=pSB1AK3
BBa_S03944	.	CDS	1158	1973	.	+	0	ID=annotation1780430;Name=kanR (rev);Parent=pSB1AK3
BBa_S03944	.	sequence_feature	1	53	.	+	0	ID=BBa_J70010;Name=BBa_J70010
BBa_S03944	.	sequence_feature	51	53	.	+	0	ID=annotation1963029;Name=PpiI2;Parent=BBa_J70010
BBa_S03944	.	sequence_feature	42	45	.	+	0	ID=annotation1963028;Name=PpiI 2;Parent=BBa_J70010
BBa_S03944	.	sequence_feature	1	3	.	+	0	ID=annotation1963026;Name=PpiI 1;Parent=BBa_J70010
BBa_S03944	.	non_covalent_binding_site	27	34	.	+	0	ID=annotation1963033;Name=PacI 2;Parent=BBa_J70010
BBa_S03944	.	non_covalent_binding_site	23	30	.	+	0	ID=annotation1963034;Name=PacI3;Parent=BBa_J70010
BBa_S03944	.	sequence_feature	35	41	.	+	0	ID=annotation1963031;Name=MabI 2;Parent=BBa_J70010
BBa_S03944	.	sequence_feature	13	19	.	+	0	ID=annotation1963030;Name=MabI 1;Parent=BBa_J70010
BBa_S03944	.	non_covalent_binding_site	19	24	.	+	0	ID=annotation1963032;Name=PacI 1;Parent=BBa_J70010
BBa_S03944	.	sequence_feature	9	12	.	+	0	ID=annotation1963027;Name=PpiI 1;Parent=BBa_J70010
>BBa_S03944
gagagctggttcaccaggttaattaattaattaaacctggtgaacgtggtctctactagagtactagtagcggccgctg
cagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgc
aggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaat
acggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaa
aaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggt
ggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccct
gccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctc
agttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccg
gtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcag
agcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggt
atctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggta
gcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctac
ggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctag
atccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgagtcccg
tcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaaaaactcatcgagcatcaaatga
aactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcac
cgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaa
tttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagc
ttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgt
tattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatg
caaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgct
gttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggca
taaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaa
caactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccattta
tacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgttgaatatggctcataa
caccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaaca
tcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagctcgagttacca
atgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtag
ataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccag
atttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtc
tattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggc
atcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatccc
ccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcact
catggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactca
accaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccac
atagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgag
atccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagca
aaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttc
aatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaat
aggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaa
aataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggc
cgcttctagag