LOCUS       BBa_S03953              1972 bp    DNA     linear       26-Dec-2024
DEFINITION  F2622:Q04400
ACCESSION   BBa_S03953
VERSION     BBa_S03953.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..83
                     /label=prefix
     misc_feature    255..255
                     /label=T
     misc_feature    573..573
                     /label=A
     CDS             82..831
                     /label=luxR
     misc_feature    82..837
                     /label=BBa_C0062
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0062
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     misc_feature    1008..1027
                     /label=LuxR/HSL
     promoter        1027..1032
                     /label=-35
     promoter        1049..1054
                     /label=-10
     misc_feature    1060..1060
                     /label=start
     misc_feature    1008..1062
                     /label=BBa_R0062
     promoter        1008..1062
                     /label=BBa_R0062
     misc_feature    1..1062
                     /label=BBa_F2622
     misc_feature    1075..1078
                     /label=conserved
     RBS             1071..1082
                     /label=BBa_B0034
     CDS             1092..1708
                     /label=tetR
     misc_feature    1709..1742
                     /label=SsrA
     misc_feature    1749..1773
                     /label=Help:Barcodes
     CDS             1089..1748
                     /label=BBa_C0040
     stem_loop       1793..1836
                     /label=stem_loop
     misc_feature    1782..1861
                     /label=BBa_B0010
     terminator      1782..1861
                     /label=BBa_B0010
     stem_loop       1877..1896
                     /label=T7 TE
     misc_feature    1903..1903
                     /label=stop
     polyA_site      1897..1910
                     /label=polya
     misc_feature    1870..1910
                     /label=BBa_B0012
     terminator      1870..1910
                     /label=BBa_B0012
     misc_feature    1919..1937
                     /label=TetR 1
     misc_feature    1919..1972
                     /label=BBa_R0040
     promoter        1938..1943
                     /label=-35
     misc_feature    1944..1962
                     /label=TetR 2
     promoter        1961..1966
                     /label=-10
     promoter        1919..1972
                     /label=BBa_R0040
     misc_feature    1071..1972
                     /label=BBa_Q04400
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt
      121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact
      181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa
      241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct
      301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat
      361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg
      421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc
      481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat
      541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat
      601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg
      661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact
      721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt
      781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac
      841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg
     1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga
     1081 aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta gagctgctta
     1141 atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc
     1201 agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg
     1261 agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt
     1321 ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa
     1381 aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag
     1441 cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg
     1501 ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa
     1561 gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc gaattatttg
     1621 atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag
     1681 aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac gctttagtag
     1741 cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc aaataaaacg
     1801 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1861 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtc
     1921 cctatcagtg atagagattg acatccctat cagtgataga gatactgagc ac
//