LOCUS BBa_S03956 1041 bp DNA linear 22-Dec-2024 DEFINITION R0079:E0240 ACCESSION BBa_S03956 VERSION BBa_S03956.1 FEATURES Location/Qualifiers misc_feature 46..63 /label=OP2 misc_feature 106..125 /label=OP1 promoter 117..122 /label=-35 promoter 140..145 /label=-10 promoter 1..157 /label=BBa_R0079 RBS 166..178 /label=RBS-3\Weak RBS 172..175 /label=RBS misc_feature 166..178 /label=BBa_B0032 RBS 166..178 /label=BBa_B0032 CDS 185..904 /label=GFP protein CDS 185..904 /label=BBa_E0040 stem_loop 924..967 /label=stem_loop misc_feature 913..992 /label=BBa_B0010 terminator 913..992 /label=BBa_B0010 stem_loop 1008..1027 /label=T7 TE misc_feature 1034..1034 /label=stop polyA_site 1028..1041 /label=polya misc_feature 1001..1041 /label=BBa_B0012 terminator 1001..1041 /label=BBa_B0012 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagtcaca caggaaagta 181 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga 241 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata 301 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac 361 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa 421 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt 481 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct 541 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca 601 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa 661 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc 721 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca 781 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt 841 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata 901 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 961 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 1021 gtgggccttt ctgcgtttat a //