BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_S03959 1 BBa_S03959 B0034:K137012 2008-07-06T11:00:00Z 2015-05-08T01:14:33Z true false _187_ 0 2987 9 Discontinued false false Victoria Hsiao component1965374 1 BBa_B0034 component1965375 1 BBa_K137012 annotation1965374 1 BBa_B0034 range1965374 1 1 12 annotation1965375 1 BBa_K137012 range1965375 1 19 1095 BBa_K137012 1 BBa_K137012 folP (dihydropteroate synthase) 2008-06-19T11:00:00Z 2015-05-08T01:10:08Z L.lactis genome folP is part of the L.lactis folate gene cluster and encodes dihydropteroate synthase. true false _187_ 0 2987 9 Discontinued false We were unsure of whether or not the L.lactis RBS would work with E. coli and so we are going to try cloning the entire gene cluster with the RBSs as well as cloning out each gene separately and adding E.coli RBSs. false Victoria Hsiao BBa_B0034_sequence 1 aaagaggagaaa BBa_K137012_sequence 1 atgaaaatcttagaacttaatcaagaatctttttctttaaaaaatattgtgataaaatttgaagaattaagtaatgatgaattgacaggcttacaaaaaaagctttatagaattggaagtcttgcaaaactgagcgaaaatagtcttctagctgttttaactattgatgagttggcaaaactacttaatcatttggaaaatattgaagtaaaaaatgagcttgaactgatttataaacggcatcaaattatttggtcaggaaaaaatttcaattttgatttgactagaaaatcaatcgtttactcaattgttaatgttactcccgattccttttatgatggcaatccagataatttaaacatttctcatattcttaaaagagtagagtccgatttagaaaatggagcatctgtacttgagttaggtggaaaatcatctcgtcctggttatgcggacattagtccagaggaagaatggagcagattaaaagaacccattgctcaaattagaaagtattttccaaaagctgtcttagccgtggatacggatgaagcttatgttatgggaagggtgttagaggctggtgtcgatattattaatgatattgatggttttgacacagcggataagctaaaagtgattgaaaagtaccagccagcagtggttgccatgaataatggtcgggcaggttttaagtatgctgacaatgtttatgaagaacttcctttattttttaaagataaaaaaatcgaactgactcagcttggaatgaatcctgaaaaaatttctattgaccccggagttggttttttcaatggagattcaggctctgatagtcttcaacgtgttaaaacaacggaacttttgagcagacttggtctgccggttatgattgcgatttctcgcaagtcatttatgggaagactcttcaatgctcaaggagaagagcgacttttttcaagtttgatgttagagggacaaatggttgctgatggtggacgtatccttagagttcatgatgtgaaagaaacgaaacgtttgcttgacgcaattgaaatttataaggaattttaataa BBa_S03959_sequence 1 aaagaggagaaatactagatgaaaatcttagaacttaatcaagaatctttttctttaaaaaatattgtgataaaatttgaagaattaagtaatgatgaattgacaggcttacaaaaaaagctttatagaattggaagtcttgcaaaactgagcgaaaatagtcttctagctgttttaactattgatgagttggcaaaactacttaatcatttggaaaatattgaagtaaaaaatgagcttgaactgatttataaacggcatcaaattatttggtcaggaaaaaatttcaattttgatttgactagaaaatcaatcgtttactcaattgttaatgttactcccgattccttttatgatggcaatccagataatttaaacatttctcatattcttaaaagagtagagtccgatttagaaaatggagcatctgtacttgagttaggtggaaaatcatctcgtcctggttatgcggacattagtccagaggaagaatggagcagattaaaagaacccattgctcaaattagaaagtattttccaaaagctgtcttagccgtggatacggatgaagcttatgttatgggaagggtgttagaggctggtgtcgatattattaatgatattgatggttttgacacagcggataagctaaaagtgattgaaaagtaccagccagcagtggttgccatgaataatggtcgggcaggttttaagtatgctgacaatgtttatgaagaacttcctttattttttaaagataaaaaaatcgaactgactcagcttggaatgaatcctgaaaaaatttctattgaccccggagttggttttttcaatggagattcaggctctgatagtcttcaacgtgttaaaacaacggaacttttgagcagacttggtctgccggttatgattgcgatttctcgcaagtcatttatgggaagactcttcaatgctcaaggagaagagcgacttttttcaagtttgatgttagagggacaaatggttgctgatggtggacgtatccttagagttcatgatgtgaaagaaacgaaacgtttgcttgacgcaattgaaatttataaggaattttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z