BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_S03960 1 BBa_S03960 B0034:K137013 2008-07-06T11:00:00Z 2015-05-08T01:14:33Z true false _187_ 0 2987 9 Discontinued false false Victoria Hsiao component1965377 1 BBa_B0034 component1965380 1 BBa_K137013 annotation1965380 1 BBa_K137013 range1965380 1 19 1305 annotation1965377 1 BBa_B0034 range1965377 1 1 12 BBa_K137013 1 BBa_K137013 folC (folylpolyglutamate synthase + dihydrofolate synthase) 2008-06-19T11:00:00Z 2015-05-08T01:10:08Z L.lactis genome folC is a necessary gene in the L.lactis folate biosynthesis gene cluster. folC is a bifunctional gene which encodes both folylpolyglutamate synthase and dihydrofolate synthase. These enzymes are needed for the last steps of dihydrofolate production. true false _187_ 0 2987 9 Discontinued false We were unsure of whether or not the L.lactis RBS would work with E. coli and so we are going to try cloning the entire gene cluster with the RBSs as well as cloning out each gene separately and adding E.coli RBSs. false Victoria Hsiao annotation1963846 1 EcoR1 site range1963846 1 979 979 annotation1963845 1 EcoR1 site range1963845 1 860 860 BBa_S03960_sequence 1 aaagaggagaaatactagatgtctattgaacaagcattagaatggatacattcacgattaaaatttaatattcgcccagggctaaatcgagtttcagcacttttagaattattaggcaatccagaggaatctttatcaatgattcacattgctggaacaaatgggaaaggatcaactgttgcctttacgcgctctatttttatgcaagctggcttaaaagttgcttcttttacgagtccttttattacgacttttggtgaacgaatgtctataaatgctcaaccaattcctgatgaaaagttaatttattatgtaaagatgattcaacctttagttgagcagcttgataatgatgctgagttgactggaattactgaatttgaaattattaccgcgatggcttttaaatattttgctgatgaaaaggttgatttggcggtaatcgaggttggcttgggtggactgcttgattcgacaaatgtcattagacccgttgtttctggaatcacaacaatcgggctagaccatattgatatcttgggttcaacgattgaagaaatcgcagctcaaaaagcaggtattattaaagctggaatacctgttgtggttggaaacattgaattaaaagctttgcgagttatttgggaagttgccagaaaaaatactgcgcgtatttatcagtatccttatgattaccgcaccgagttaaaagagcatgaacattttgatttctttgcaggtcaagaggcaattctggatttggaaaaatctttgattggcttgcatcaagtagaaaatgctggaatggccattgaattggcgctcgtctatgcgaataaagttggaattaacttgaatgaagagattattcgtgcgggaattcgtgaagctttttggcctgctcgaatggaaaaattaggcgaaaatcctttaattttacttgatggggctcataatgttcatgctatgaatcgtttgcttgagaacttggggaaagaattccctgataaaaaaattacaattattttttcagctattaccacaaaagatattaggcagatgataaaaatgcttcaaacggtaaaaaattctcgcttaattttgacaacttttgattatcctaaagctttagacttgaaagattttcggtatttagaggatgaaggggttgaattagcaccaagttgggaattagcgattgttcgtgcacaaaaagctttagatgaagatgagctcctattaatcactggttcgctctatttttcatcacaagttcgtgaatttttgaaaaaagaaaagtaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K137013_sequence 1 atgtctattgaacaagcattagaatggatacattcacgattaaaatttaatattcgcccagggctaaatcgagtttcagcacttttagaattattaggcaatccagaggaatctttatcaatgattcacattgctggaacaaatgggaaaggatcaactgttgcctttacgcgctctatttttatgcaagctggcttaaaagttgcttcttttacgagtccttttattacgacttttggtgaacgaatgtctataaatgctcaaccaattcctgatgaaaagttaatttattatgtaaagatgattcaacctttagttgagcagcttgataatgatgctgagttgactggaattactgaatttgaaattattaccgcgatggcttttaaatattttgctgatgaaaaggttgatttggcggtaatcgaggttggcttgggtggactgcttgattcgacaaatgtcattagacccgttgtttctggaatcacaacaatcgggctagaccatattgatatcttgggttcaacgattgaagaaatcgcagctcaaaaagcaggtattattaaagctggaatacctgttgtggttggaaacattgaattaaaagctttgcgagttatttgggaagttgccagaaaaaatactgcgcgtatttatcagtatccttatgattaccgcaccgagttaaaagagcatgaacattttgatttctttgcaggtcaagaggcaattctggatttggaaaaatctttgattggcttgcatcaagtagaaaatgctggaatggccattgaattggcgctcgtctatgcgaataaagttggaattaacttgaatgaagagattattcgtgcgggaattcgtgaagctttttggcctgctcgaatggaaaaattaggcgaaaatcctttaattttacttgatggggctcataatgttcatgctatgaatcgtttgcttgagaacttggggaaagaattccctgataaaaaaattacaattattttttcagctattaccacaaaagatattaggcagatgataaaaatgcttcaaacggtaaaaaattctcgcttaattttgacaacttttgattatcctaaagctttagacttgaaagattttcggtatttagaggatgaaggggttgaattagcaccaagttgggaattagcgattgttcgtgcacaaaaagctttagatgaagatgagctcctattaatcactggttcgctctatttttcatcacaagttcgtgaatttttgaaaaaagaaaagtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z