LOCUS       BBa_S03969               971 bp    DNA     linear       15-Jun-2024
DEFINITION  pMnt/Lac-RBS-GFP-TT
ACCESSION   BBa_S03969
VERSION     BBa_S03969.1  
FEATURES             Location/Qualifiers
     promoter        47..52
                     /label=-10
     misc_feature    53..87
                     /label=LacI
     misc_feature    1..52
                     /label=Mnt
     promoter        1..87
                     /label=BBa_K091104
     RBS             96..108
                     /label=RBS-3\Weak
     RBS             102..105
                     /label=RBS
     misc_feature    96..108
                     /label=BBa_B0032
     RBS             96..108
                     /label=BBa_B0032
     CDS             115..834
                     /label=GFP protein
     CDS             115..834
                     /label=BBa_E0040
     stem_loop       854..897
                     /label=stem_loop
     misc_feature    843..922
                     /label=BBa_B0010
     terminator      843..922
                     /label=BBa_B0010
     stem_loop       938..957
                     /label=T7 TE
     misc_feature    964..964
                     /label=stop
     polyA_site      958..971
                     /label=polya
     misc_feature    931..971
                     /label=BBa_B0012
     terminator      931..971
                     /label=BBa_B0012
ORIGIN
        1 ctcgaggtga gtgcacagta ctaggtccac ggtgacctag atctcctata gttgtgtgga
       61 attgtgagcg gataacaatt tcacacatac tagagtcaca caggaaagta ctagatgcgt
      121 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
      181 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
      241 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
      301 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac
      361 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
      421 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
      481 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
      541 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
      601 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
      661 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
      721 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
      781 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag
      841 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      901 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
      961 ctgcgtttat a
//