LOCUS       BBa_S03972              3230 bp    DNA     linear       14-May-2024
DEFINITION  B0015:I732017
ACCESSION   BBa_S03972
VERSION     BBa_S03972.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     misc_feature    142..145
                     /label=conserved
     RBS             138..149
                     /label=BBa_B0034
     misc_feature    3179..3179
                     /label=C->T
     CDS             156..3230
                     /label=lacZ
     misc_feature    156..158
                     /label=START
     misc_feature    3228..3230
                     /label=STOP
     CDS             156..3230
                     /label=BBa_I732005
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagaaa gaggagaaat actagatgac catgattacg gattcactgg
      181 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      241 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      301 cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag
      361 cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct
      421 caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtgacc tatcccatta
      481 cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta
      541 atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact
      601 cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc
      661 cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg
      721 tgctgcgctg gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca
      781 ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg
      841 ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg
      901 gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg
      961 ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc
     1021 gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc
     1081 tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct
     1141 gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc
     1201 cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg
     1261 atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc
     1321 gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt
     1381 atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg
     1441 atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc
     1501 gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc
     1561 acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag
     1621 gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg
     1681 aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg
     1741 gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg
     1801 gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct
     1861 gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt
     1921 acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct
     1981 ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc
     2041 agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg
     2101 ataacgagct cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag
     2161 tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc
     2221 cggagagcgc cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat
     2281 ggtcagaagc cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg
     2341 tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt
     2401 gcatcgagct gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga
     2461 tgtggattgg cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac
     2521 cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg
     2581 aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag
     2641 atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa
     2701 ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg
     2761 ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc
     2821 tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg
     2881 accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc
     2941 cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc
     3001 cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga
     3061 tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg
     3121 gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaatttc
     3181 agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaataa
//