LOCUS       BBa_S03984              1010 bp    DNA     linear       22-Dec-2024
DEFINITION  K091146:E0240
ACCESSION   BBa_S03984
VERSION     BBa_S03984.1  
FEATURES             Location/Qualifiers
     misc_feature    75..93
                     /label=LasR
     misc_feature    94..113
                     /label=LuxR
     misc_feature    113..118
                     /label=-10
     misc_feature    86..91
                     /label=-35
     misc_feature    125..125
                     /label=Transcriptional Start
     promoter        1..126
                     /label=BBa_K091146
     RBS             135..147
                     /label=RBS-3\Weak
     RBS             141..144
                     /label=RBS
     misc_feature    135..147
                     /label=BBa_B0032
     RBS             135..147
                     /label=BBa_B0032
     CDS             154..873
                     /label=GFP protein
     CDS             154..873
                     /label=BBa_E0040
     stem_loop       893..936
                     /label=stem_loop
     misc_feature    882..961
                     /label=BBa_B0010
     terminator      882..961
                     /label=BBa_B0010
     stem_loop       977..996
                     /label=T7 TE
     misc_feature    1003..1003
                     /label=stop
     polyA_site      997..1010
                     /label=polya
     misc_feature    970..1010
                     /label=BBa_B0012
     terminator      970..1010
                     /label=BBa_B0012
ORIGIN
        1 tgttctcgtg tgaagccatt gctctgatct tttggacgtt tcttcgagcc tagcaagggt
       61 ccgggttcac cgaaatctat ctcatttgct agtacctgta ggatcgtaca ggtataaatt
      121 cttcagtact agagtcacac aggaaagtac tagatgcgta aaggagaaga acttttcact
      181 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      241 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
      301 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa
      361 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc
      421 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
      481 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat
      541 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat
      601 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac
      661 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc
      721 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
      781 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
      841 acacatggca tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg
      901 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      961 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//