LOCUS       BBa_S03985              1954 bp    DNA     linear       23-Dec-2024
DEFINITION  S03954:S03981
ACCESSION   BBa_S03985
VERSION     BBa_S03985.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..735
                     /label=lasR
     misc_feature    736..768
                     /label=LVA
     misc_feature    498..498
                     /label=G
     misc_feature    775..799
                     /label=Help:Barcodes
     CDS             19..774
                     /label=BBa_C0079
     misc_feature    1..799
                     /label=BBa_S03156
     stem_loop       819..862
                     /label=stem_loop
     misc_feature    808..887
                     /label=BBa_B0010
     terminator      808..887
                     /label=BBa_B0010
     stem_loop       903..922
                     /label=T7 TE
     misc_feature    929..929
                     /label=stop
     polyA_site      923..936
                     /label=polya
     misc_feature    896..936
                     /label=BBa_B0012
     terminator      896..936
                     /label=BBa_B0012
     terminator      808..936
                     /label=BBa_B0015
     misc_feature    1..936
                     /label=BBa_S03954
     misc_feature    1030..1035
                     /label=-35 Region
     misc_feature    1019..1037
                     /label=LasR Binding Site
     misc_feature    1057..1062
                     /label= -10 Region
     misc_feature    1069..1069
                     /label=Primary Transcriptional Start
     promoter        945..1070
                     /label=BBa_K091117
     RBS             1079..1091
                     /label=RBS-3\Weak
     RBS             1085..1088
                     /label=RBS
     misc_feature    1079..1091
                     /label=BBa_B0032
     RBS             1079..1091
                     /label=BBa_B0032
     CDS             1098..1817
                     /label=GFP protein
     CDS             1098..1817
                     /label=BBa_E0040
     stem_loop       1837..1880
                     /label=stem_loop
     misc_feature    1826..1905
                     /label=BBa_B0010
     terminator      1826..1905
                     /label=BBa_B0010
     stem_loop       1921..1940
                     /label=T7 TE
     misc_feature    1947..1947
                     /label=stop
     polyA_site      1941..1954
                     /label=polya
     misc_feature    1914..1954
                     /label=BBa_B0012
     terminator      1914..1954
                     /label=BBa_B0012
     misc_feature    945..1954
                     /label=BBa_S03981
ORIGIN
        1 aaagaggaga aatactagat ggccttggtt gacggttttc ttgagctgga acgctcaagt
       61 ggaaaattgg agtggagcgc catcctccag aagatggcga gcgaccttgg attctcgaag
      121 atcctgttcg gcctgttgcc taaggacagc caggactacg agaacgcctt catcgtcggc
      181 aactacccgg ccgcctggcg cgagcattac gaccgggctg gctacgcgcg ggtcgacccg
      241 acggtcagtc actgtaccca gagcgtactg ccgattttct gggaaccgtc catctaccag
      301 acgcgaaagc agcacgagtt cttcgaggaa gcctcggccg ccggcctggt gtatgggctg
      361 accatgccgc tgcatggtgc tcgcggcgaa ctcggcgcgc tgagcctcag cgtggaagcg
      421 gaaaaccggg ccgaggccaa ccgtttcata gagtcggtcc tgccgaccct gtggatgctc
      481 aaggactacg cactgcaaag cggtgccgga ctggccttcg aacatccggt cagcaaaccg
      541 gtggttctga ccagccggga gaaggaagtg ttgcagtggt gcgccatcgg caagaccagt
      601 tgggagatat cggttatctg caactgctcg gaagccaatg tgaacttcca tatgggaaat
      661 attcggcgga agttcggtgt gacctcccgc cgcgtagcgg ccattatggc cgttaatttg
      721 ggtcttatta ctctcgctgc aaacgacgaa aactacgctt tagtagctta ataacactga
      781 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      901 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtgttct cgtgtgaagc
      961 cattgctctg atcttttgga cgtttcttcg agcctagcaa gggtccgggt tcaccgaaat
     1021 ctatctcatt tgctagttat aaaattatga aatttgtata aattcttcag tactagagtc
     1081 acacaggaaa gtactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc
     1141 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
     1201 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
     1261 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc
     1321 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
     1381 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
     1441 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
     1501 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
     1561 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
     1621 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     1681 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
     1741 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
     1801 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1861 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1921 ggctcacctt cgggtgggcc tttctgcgtt tata
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