##gff-version 3
##sequence-region BBa_S03985 1 1954
BBa_S03985	.	engineered_region	945	1954	.	+	0	ID=BBa_S03981;Name=BBa_S03981
BBa_S03985	.	ribosome_entry_site	1079	1091	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S03981
BBa_S03985	.	engineered_region	1079	1091	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S03985	.	ribosome_entry_site	1079	1091	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S03985	.	ribosome_entry_site	1085	1088	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S03985	.	CDS	1098	1817	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_S03981
BBa_S03985	.	CDS	1098	1817	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_S03985	.	terminator	1826	1905	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03981
BBa_S03985	.	stem_loop	1837	1880	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03985	.	engineered_region	1826	1905	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03985	.	promoter	945	1070	.	+	0	ID=BBa_K091117;Name=BBa_K091117;Parent=BBa_S03981
BBa_S03985	.	non_covalent_binding_site	1019	1037	.	+	0	ID=annotation1964073;Name=LasR Binding Site;Parent=BBa_K091117
BBa_S03985	.	sequence_feature	1030	1035	.	+	0	ID=annotation1964072;Name=-35 Region;Parent=BBa_K091117
BBa_S03985	.	start_codon	1069	1069	.	+	0	ID=annotation1964075;Name=Primary Transcriptional Start;Parent=BBa_K091117
BBa_S03985	.	sequence_feature	1057	1062	.	+	0	ID=annotation1964074;Name= -10 Region;Parent=BBa_K091117
BBa_S03985	.	terminator	1914	1954	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03981
BBa_S03985	.	polyA_site	1941	1954	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03985	.	engineered_region	1914	1954	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03985	.	stem_loop	1921	1940	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03985	.	stop_codon	1947	1947	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03985	.	engineered_region	1	936	.	+	0	ID=BBa_S03954;Name=BBa_S03954
BBa_S03985	.	terminator	808	936	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S03954
BBa_S03985	.	terminator	896	936	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S03985	.	polyA_site	923	936	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S03985	.	engineered_region	896	936	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S03985	.	stem_loop	903	922	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S03985	.	stop_codon	929	929	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S03985	.	terminator	808	887	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S03985	.	stem_loop	819	862	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S03985	.	engineered_region	808	887	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S03985	.	engineered_region	1	799	.	+	0	ID=BBa_S03156;Name=BBa_S03156;Parent=BBa_S03954
BBa_S03985	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03156
BBa_S03985	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S03985	.	CDS	19	774	.	+	0	ID=BBa_C0079;Name=BBa_C0079;Parent=BBa_S03156
BBa_S03985	.	engineered_tag	775	799	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_S03985	.	tag	736	768	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_S03985	.	CDS	19	735	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_S03985	.	sequence_alteration	498	498	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
>BBa_S03985
aaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcg
ccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggacta
cgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgac
ccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacg
agttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcgg
cgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtgg
atgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgacca
gccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcgga
agccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggcc
gttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagt
gtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtgtt
ctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctc
atttgctagttataaaattatgaaatttgtataaattcttcagtactagagtcacacaggaaagtactagatgcgtaaa
ggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtca
gtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttcc
atggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgac
tttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacac
gtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaa
cattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatc
aaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaa
ttggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaa
gagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
tactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtg
aacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata