LOCUS       BBa_S03986              1954 bp    DNA     linear       15-Dec-2024
DEFINITION  I0462:S03981
ACCESSION   BBa_S03986
VERSION     BBa_S03986.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..20
                     /label=prefix
     misc_feature    192..192
                     /label=T
     misc_feature    510..510
                     /label=A
     CDS             19..768
                     /label=luxR
     misc_feature    19..774
                     /label=BBa_C0062
     misc_feature    775..799
                     /label=Help:Barcodes
     CDS             19..774
                     /label=BBa_C0062
     stem_loop       819..862
                     /label=stem_loop
     misc_feature    808..887
                     /label=BBa_B0010
     terminator      808..887
                     /label=BBa_B0010
     stem_loop       903..922
                     /label=T7 TE
     misc_feature    929..929
                     /label=stop
     polyA_site      923..936
                     /label=polya
     misc_feature    896..936
                     /label=BBa_B0012
     terminator      896..936
                     /label=BBa_B0012
     misc_feature    1..936
                     /label=BBa_I0462
     misc_feature    1030..1035
                     /label=-35 Region
     misc_feature    1019..1037
                     /label=LasR Binding Site
     misc_feature    1057..1062
                     /label= -10 Region
     misc_feature    1069..1069
                     /label=Primary Transcriptional Start
     promoter        945..1070
                     /label=BBa_K091117
     RBS             1079..1091
                     /label=RBS-3\Weak
     RBS             1085..1088
                     /label=RBS
     misc_feature    1079..1091
                     /label=BBa_B0032
     RBS             1079..1091
                     /label=BBa_B0032
     CDS             1098..1817
                     /label=GFP protein
     CDS             1098..1817
                     /label=BBa_E0040
     stem_loop       1837..1880
                     /label=stem_loop
     misc_feature    1826..1905
                     /label=BBa_B0010
     terminator      1826..1905
                     /label=BBa_B0010
     stem_loop       1921..1940
                     /label=T7 TE
     misc_feature    1947..1947
                     /label=stop
     polyA_site      1941..1954
                     /label=polya
     misc_feature    1914..1954
                     /label=BBa_B0012
     terminator      1914..1954
                     /label=BBa_B0012
     misc_feature    945..1954
                     /label=BBa_S03981
ORIGIN
        1 aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag aataattaat
       61 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa
      121 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct
      181 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat
      241 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg
      301 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa
      361 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga
      421 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg
      481 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata
      541 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca
      601 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc
      661 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt
      721 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga
      781 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      901 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtgttct cgtgtgaagc
      961 cattgctctg atcttttgga cgtttcttcg agcctagcaa gggtccgggt tcaccgaaat
     1021 ctatctcatt tgctagttat aaaattatga aatttgtata aattcttcag tactagagtc
     1081 acacaggaaa gtactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc
     1141 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
     1201 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
     1261 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc
     1321 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
     1381 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
     1441 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
     1501 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
     1561 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
     1621 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     1681 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
     1741 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
     1801 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1861 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1921 ggctcacctt cgggtgggcc tttctgcgtt tata
//