LOCUS       BBa_S04012              3273 bp    DNA     linear       14-May-2024
DEFINITION  J23100:S03973
ACCESSION   BBa_S04012
VERSION     BBa_S04012.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    3085..3085
                     /label=C->T
     CDS             62..3136
                     /label=lacZ
     misc_feature    62..64
                     /label=START
     misc_feature    3134..3136
                     /label=STOP
     CDS             62..3136
                     /label=BBa_I732005
     stem_loop       3156..3199
                     /label=stem_loop
     misc_feature    3145..3224
                     /label=BBa_B0010
     terminator      3145..3224
                     /label=BBa_B0010
     stem_loop       3240..3259
                     /label=T7 TE
     misc_feature    3266..3266
                     /label=stop
     polyA_site      3260..3273
                     /label=polya
     misc_feature    3233..3273
                     /label=BBa_B0012
     terminator      3233..3273
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaccatg attacggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      121 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      181 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      241 ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt gcgatcttcc
      301 tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg atgcgcccat
      361 ctacaccaac gtgacctatc ccattacggt caatccgccg tttgttccca cggagaatcc
      421 gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg aaggccagac
      481 gcgaattatt tttgatggcg ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt
      541 cggttacggc caggacagtc gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc
      601 cggagaaaac cgcctcgcgg tgatggtgct gcgctggagt gacggcagtt atctggaaga
      661 tcaggatatg tggcggatga gcggcatttt ccgtgacgtc tcgttgctgc ataaaccgac
      721 tacacaaatc agcgatttcc atgttgccac tcgctttaat gatgatttca gccgcgctgt
      781 actggaggct gaagttcaga tgtgcggcga gttgcgtgac tacctacggg taacagtttc
      841 tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg cctttcggcg gtgaaattat
      901 cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt ctgaacgtcg aaaacccgaa
      961 actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg gttgaactgc acaccgccga
     1021 cggcacgctg attgaagcag aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa
     1081 tggtctgctg ctgctgaacg gcaagccgtt gctgattcga ggcgttaacc gtcacgagca
     1141 tcatcctctg catggtcagg tcatggatga gcagacgatg gtgcaggata tcctgctgat
     1201 gaagcagaac aactttaacg ccgtgcgctg ttcgcattat ccgaaccatc cgctgtggta
     1261 cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa gccaatattg aaacccacgg
     1321 catggtgcca atgaatcgtc tgaccgatga tccgcgctgg ctaccggcga tgagcgaacg
     1381 cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg
     1441 gaatgaatca ggccacggcg ctaatcacga cgcgctgtat cgctggatca aatctgtcga
     1501 tccttcccgc ccggtgcagt atgaaggcgg cggagccgac accacggcca ccgatattat
     1561 ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc
     1621 catcaaaaaa tggctttcgc tacctggaga gacgcgcccg ctgatccttt gcgaatacgc
     1681 ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta
     1741 tccccgttta cagggcggct tcgtctggga ctgggtggat cagtcgctga ttaaatatga
     1801 tgaaaacggc aacccgtggt cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg
     1861 ccagttctgt atgaacggtc tggtctttgc cgaccgcacg ccgcatccag cgctgacgga
     1921 agcaaaacac cagcagcagt ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac
     1981 cagcgaatac ctgttccgtc atagcgataa cgagctcctg cactggatgg tggcgctgga
     2041 tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc gctccacaag gtaaacagtt
     2101 gattgaactg cctgaactac cgcagccgga gagcgccggg caactctggc tcacagtacg
     2161 cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg cacatcagcg cctggcagca
     2221 gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc gcgtcccacg ccatcccgca
     2281 tctgaccacc agcgaaatgg atttttgcat cgagctgggt aataagcgtt ggcaatttaa
     2341 ccgccagtca ggctttcttt cacagatgtg gattggcgat aaaaaacaac tgctgacgcc
     2401 gctgcgcgat cagttcaccc gtgcaccgct ggataacgac attggcgtaa gtgaagcgac
     2461 ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg gcgggccatt accaggccga
     2521 agcagcgttg ttgcagtgca cggcagatac acttgctgat gcggtgctga ttacgaccgc
     2581 tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc cggaaaacct accggattga
     2641 tggtagtggt caaatggcga ttaccgttga tgttgaagtg gcgagcgata caccgcatcc
     2701 ggcgcggatt ggcctgaact gccagctggc gcaggtagca gagcgggtaa actggctcgg
     2761 attagggccg caagaaaact atcccgaccg ccttactgcc gcctgttttg accgctggga
     2821 tctgccattg tcagacatgt ataccccgta cgtcttcccg agcgaaaacg gtctgcgctg
     2881 cgggacgcgc gaattgaatt atggcccaca ccagtggcgc ggcgacttcc agttcaacat
     2941 cagccgctac agtcaacagc aactgatgga aaccagccat cgccatctgc tgcacgcgga
     3001 agaaggcaca tggctgaata tcgacggttt ccatatgggg attggtggcg acgactcctg
     3061 gagcccgtca gtatcggcgg aatttcagct gagcgccggt cgctaccatt accagttggt
     3121 ctggtgtcaa aaataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     3181 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     3241 gctcaccttc gggtgggcct ttctgcgttt ata
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