BBa_S04033 1 folKE +j23 J23100:S03958 2008-07-27T11:00:00Z 2015-05-08T01:14:34Z false false _187_ 0 2987 9 It's complicated false false Victoria Hsiao component1969059 1 BBa_K137011 component1969058 1 BBa_B0034 component1969056 1 BBa_J23100 annotation1969056 1 BBa_J23100 range1969056 1 1 35 annotation1969059 1 BBa_K137011 range1969059 1 62 1114 annotation1969058 1 BBa_B0034 range1969058 1 44 55 BBa_K137011 1 folKE folKE (GTP Cyclohydrolase I + pyrophosphokinase) 2008-06-19T11:00:00Z 2015-05-08T01:10:08Z L.lactis genome folKE is a bifunctional gene which encodes two necessary enzymes for folate biosynthesis (2-Amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase / GTP cyclohydrolase I). false false _187_ 0 2987 9 It's complicated false We were unsure of whether or not the L.lactis RBS would work with E. coli and so we are going to try cloning the entire gene cluster with the RBSs as well as cloning out each gene separately and adding E.coli RBSs. false Victoria Hsiao BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0034_sequence 1 aaagaggagaaa BBa_S04033_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgcaaacaacttatttaagcatgggaagtaatattggtgaccgtcagtattatttacatgaagccattcgtttattgggaaaacaccctaaaattatgattgaaaaagtatcaaatttttatgaaagtactccagtcggcggcgtcaaacaagatgattttactaatttggcattaaaggtggcaacgctacttgaacctttggaattattatcttttattcatgaagttgagctatctttgaaccgtgagcgaaaaattcattgggggccaagaacaattgatattgatattattttctataacgacttagaaatgcaagaagaaaacttggttattccacataaagaagcttttaatcgtctttttgtcttgaaacctatttttgaacttattgataaagactttaaatattatgcgtcaatagaaaaagcaatagccgaactttcagtaagtgaacaagagctccatgtgataaaagaagaaaaaacaccaagaaatcgtattgaagatgctgttaaagagattctctttgcagtaggtgaaaatccaaatcgagaaggattacttgaaactccagcgagagtagctaaaatgtatgaagaaattctttcgtcacaacgcttaagcaagtttaatgagtataaactttttgaaattgattcttctaaaacggattcaatcgtgttgattaaagatattcctttttattcaatgtgtgagcatcatatgttaccattttttgggaaagctcatgttgcctatattccagctgatggaaaaattattggcttgtcaaaaattccccgtttagttgattatgtttcgcgcaaactctcagttcaagaaaatatcactcatgatattggagatgttttgactgatattttgaatcctaaaggagtggcagttcttgttgaaggacgtcatatgtgcgttgaaatgcgtggagtaaaaaaagtaaattctattactaaaacttcttattttttaggtgaatttaaagaaaataatgaaaaaagaatggaatttttagaaagtcttttataataa BBa_K137011_sequence 1 atgcaaacaacttatttaagcatgggaagtaatattggtgaccgtcagtattatttacatgaagccattcgtttattgggaaaacaccctaaaattatgattgaaaaagtatcaaatttttatgaaagtactccagtcggcggcgtcaaacaagatgattttactaatttggcattaaaggtggcaacgctacttgaacctttggaattattatcttttattcatgaagttgagctatctttgaaccgtgagcgaaaaattcattgggggccaagaacaattgatattgatattattttctataacgacttagaaatgcaagaagaaaacttggttattccacataaagaagcttttaatcgtctttttgtcttgaaacctatttttgaacttattgataaagactttaaatattatgcgtcaatagaaaaagcaatagccgaactttcagtaagtgaacaagagctccatgtgataaaagaagaaaaaacaccaagaaatcgtattgaagatgctgttaaagagattctctttgcagtaggtgaaaatccaaatcgagaaggattacttgaaactccagcgagagtagctaaaatgtatgaagaaattctttcgtcacaacgcttaagcaagtttaatgagtataaactttttgaaattgattcttctaaaacggattcaatcgtgttgattaaagatattcctttttattcaatgtgtgagcatcatatgttaccattttttgggaaagctcatgttgcctatattccagctgatggaaaaattattggcttgtcaaaaattccccgtttagttgattatgtttcgcgcaaactctcagttcaagaaaatatcactcatgatattggagatgttttgactgatattttgaatcctaaaggagtggcagttcttgttgaaggacgtcatatgtgcgttgaaatgcgtggagtaaaaaaagtaaattctattactaaaacttcttattttttaggtgaatttaaagaaaataatgaaaaaagaatggaatttttagaaagtcttttataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z