BBa_K137006
1
BBa_K137006
pabB (from pABA synthesis)
2008-06-19T11:00:00Z
2015-05-08T01:10:08Z
L.lactis genome
PabB from L.lactis is a fusion gene of both the usual pabB and pabC in the pABA synthesis pathway from chorismate.
false
false
_187_
0
2987
9
It's complicated
false
The start codon of pabB in L.lactis is TTG, which cannot be used in E.coli. So we designed the primer such that the first T was omitted and the A from the xba1 primer make an ATG start codon.
false
Victoria Hsiao
annotation1971718
1
Changed T to A
range1971718
1
1
1
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_S04039
1
BBa_S04039
J23100:S03977
2008-07-28T11:00:00Z
2015-05-08T01:14:34Z
false
false
_187_
0
2987
9
It's complicated
false
false
Victoria Hsiao
component1971736
1
BBa_K137006
component1971733
1
BBa_J23100
component1971735
1
BBa_B0034
annotation1971733
1
BBa_J23100
range1971733
1
1
35
annotation1971735
1
BBa_B0034
range1971735
1
44
55
annotation1971736
1
BBa_K137006
range1971736
1
62
1951
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_K137006_sequence
1
atgaaggaatttataataaaaaatacggatatttggaaaattttcctaaaatattatcggtcagatgaagaaattgtttttttacatagtagtcaggtgaccgaaaatgagcattatagtattttagctcataaaccttataaaaaggtttcaaaatataagggtcaagtgttttttaatggtgaaaaaaagaagttcaattttttagatgcggtagatttgctgaaggatgagagagttgaacgacctaaaaattggcctttttatccagaattattgggttttgtaagttatgagcaagatccggcttgttttgcagcttatgatgaagttttactttttgaccatagaacaaaactgttacgtgtcgtgcaatttgaacaaactgatggccaatattggctgactgaatcggaagaaattgaagtggattcagagattgagttcgatgttcagaatggcataggggctgtttttattgaccaaacgaggcaagaatatattgcttcaataaaaaaaatacaagattatatgaaggctggcgatatttatgttgccaatttgacgcaacaatttgaaatttggtccgatcaaaaacctattgatgtttttaagaaaacaaggaaacaaattcctgctcctttctcatcatttttacaatatcctgaatggaaaatgacgcaaatttcaagttctgttgaacgctttgtttctattcataatggagcattaatttcaaaacccattaaaggaacaattgccagaggagaaaatgttgtagctgatagattacaaaagaaaatactttccgacagtagtaaagaacggtctgagttactgatggtgacggatttattgcgtaatgatattgcccgaattagtcaacctttttctttgtctgtccctaaatttgcagaaattgaaactttttctcatgttcatcaactggtgacttcaataaaaagtagaattaaagaagatttgactttttcagaatttatgacggctttatttccgggaggttcaatcacaggtacacctaagaagcgggcaatggagattattaaggaagttgaaaaacaacctagaggaatttatacagggatgcagggttggttaagtcgagaaatggatttggatatgaacatcgtgattcggacattagttcatgacggtgagcattatcagcttggtgtaggcggtggaattacgttcgagagcgaagcagaagcagagttttcagagattttactaaaagcaaaaccctttttagatatattaggcctgaaagatgttccaagtattttatttacaacaggccttgttaaaaatggtgaattgttaaatttagagggtcatgtcaatcgcttgaaaaaacaataccatcatgcagatttggaagaaaaattgagaatatttgcccaaaaggttactgacggggttttacgtatcagtactgacggagattctttgacccctgggattcgtcagttgacccattcaaatgaggcctatcgagttaaactgtcgtcaattaatgacaaacctagtcttttgtctaactttaaactttcaggcccagattttcaaaaggtctttcgtcaggaagtcttagaagcgaaaaaagaagggtttcaagatattctatttcatacagatggtttgatcagtgaattatcaatcggaaattttgtggcaaaaaaggggaatcagtacgaaacgccagccaaatatgctctaaaaggaacttttttagatttatttgcgaaaaatcatacgctaatatataaagatattgcgatttctgatttgaaaacttacgatcgtttttatatgacaaatgctgtcagaggattagttgaaataaaaattgatggaatttcctaataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_S04039_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaaggaatttataataaaaaatacggatatttggaaaattttcctaaaatattatcggtcagatgaagaaattgtttttttacatagtagtcaggtgaccgaaaatgagcattatagtattttagctcataaaccttataaaaaggtttcaaaatataagggtcaagtgttttttaatggtgaaaaaaagaagttcaattttttagatgcggtagatttgctgaaggatgagagagttgaacgacctaaaaattggcctttttatccagaattattgggttttgtaagttatgagcaagatccggcttgttttgcagcttatgatgaagttttactttttgaccatagaacaaaactgttacgtgtcgtgcaatttgaacaaactgatggccaatattggctgactgaatcggaagaaattgaagtggattcagagattgagttcgatgttcagaatggcataggggctgtttttattgaccaaacgaggcaagaatatattgcttcaataaaaaaaatacaagattatatgaaggctggcgatatttatgttgccaatttgacgcaacaatttgaaatttggtccgatcaaaaacctattgatgtttttaagaaaacaaggaaacaaattcctgctcctttctcatcatttttacaatatcctgaatggaaaatgacgcaaatttcaagttctgttgaacgctttgtttctattcataatggagcattaatttcaaaacccattaaaggaacaattgccagaggagaaaatgttgtagctgatagattacaaaagaaaatactttccgacagtagtaaagaacggtctgagttactgatggtgacggatttattgcgtaatgatattgcccgaattagtcaacctttttctttgtctgtccctaaatttgcagaaattgaaactttttctcatgttcatcaactggtgacttcaataaaaagtagaattaaagaagatttgactttttcagaatttatgacggctttatttccgggaggttcaatcacaggtacacctaagaagcgggcaatggagattattaaggaagttgaaaaacaacctagaggaatttatacagggatgcagggttggttaagtcgagaaatggatttggatatgaacatcgtgattcggacattagttcatgacggtgagcattatcagcttggtgtaggcggtggaattacgttcgagagcgaagcagaagcagagttttcagagattttactaaaagcaaaaccctttttagatatattaggcctgaaagatgttccaagtattttatttacaacaggccttgttaaaaatggtgaattgttaaatttagagggtcatgtcaatcgcttgaaaaaacaataccatcatgcagatttggaagaaaaattgagaatatttgcccaaaaggttactgacggggttttacgtatcagtactgacggagattctttgacccctgggattcgtcagttgacccattcaaatgaggcctatcgagttaaactgtcgtcaattaatgacaaacctagtcttttgtctaactttaaactttcaggcccagattttcaaaaggtctttcgtcaggaagtcttagaagcgaaaaaagaagggtttcaagatattctatttcatacagatggtttgatcagtgaattatcaatcggaaattttgtggcaaaaaaggggaatcagtacgaaacgccagccaaatatgctctaaaaggaacttttttagatttatttgcgaaaaatcatacgctaatatataaagatattgcgatttctgatttgaaaacttacgatcgtttttatatgacaaatgctgtcagaggattagttgaaataaaaattgatggaatttcctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z