LOCUS       BBa_S04051              2867 bp    DNA     linear       04-Jul-2024
DEFINITION  S03975:K137069
ACCESSION   BBa_S04051
VERSION     BBa_S04051.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..83
                     /label=prefix
     misc_feature    255..255
                     /label=T
     misc_feature    573..573
                     /label=A
     CDS             82..831
                     /label=luxR
     misc_feature    82..837
                     /label=BBa_C0062
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0062
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     misc_feature    1008..1027
                     /label=LuxR/HSL
     promoter        1027..1032
                     /label=-35
     promoter        1049..1054
                     /label=-10
     misc_feature    1060..1060
                     /label=start
     misc_feature    1008..1062
                     /label=BBa_R0062
     promoter        1008..1062
                     /label=BBa_R0062
     misc_feature    1..1062
                     /label=BBa_F2622
     misc_feature    1075..1078
                     /label=conserved
     RBS             1071..1082
                     /label=BBa_B0034
     misc_feature    1..1082
                     /label=BBa_S03975
     misc_feature    1115..1115
                     /label=T->G
     CDS             1089..2867
                     /label=BBa_K137069
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt
      121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact
      181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa
      241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct
      301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat
      361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg
      421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc
      481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat
      541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat
      601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg
      661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact
      721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt
      781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac
      841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg
     1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga
     1081 aatactagat gactcaaggg aaaattactg catcggcagc aatgcttaac gtattgaaaa
     1141 catggggcgt agatacaatc tacggtatcc catcaggaac actcagctca ttgatggacg
     1201 ctttggctga agacaaagat atccgcttct tacaagttcg ccacgaagag acaggtgctc
     1261 ttgcagcggt tatgcaagct aaattcggcg gctcaatcgg ggttgcagtt ggttcaggtg
     1321 gtccaggtgc gactcacttg attaacggtg tttacgatgc agctatggat gacactccat
     1381 tcctagcgat ccttggatca cgtccagtta acgaattgaa catggatgct ttccaagagc
     1441 ttaaccaaaa cccaatgtac aacggtatcg ctgtttacaa caaacgtgta gcttacgctg
     1501 agcaattgcc aaaagtaatt gacgaagcct gccgtgctgc aatttctaaa aaaggtccag
     1561 ctgttgttga aattccagta aacttcggtt tccaagaaat cgacgaaaac tcatactacg
     1621 gttcaggttc atacgaacgc tcattcatcg ctcctgcttt gaacgaagtt gaaatcgaca
     1681 aagctgttga aatcttgaac aatgctgaac gcccagttat ctatgctgga tttggtggtg
     1741 ttaaagctgg tgaagtgatt actgaattgt cacgtaaaat caaagcacca atcatcacaa
     1801 ctggtaaaaa ctttgaagct ttcgaatgga actatgaagg tttgacaggt tctgcttacc
     1861 gtgttggttg gaaaccagcc aacgaagtgg tctttgaagc agacacagtt cttttccttg
     1921 gttcaaactt cgcatttgct gaagtttacg aagcattcaa gaacactgaa aaattcatac
     1981 aagtcgatat cgacccttac aaacttggta aacgtcatgc ccttgacgct tcaatccttg
     2041 gtgatgctgg tcaagcagct aaagctatcc ttgacaaagt aaacccagtt gaatcaactc
     2101 catggtggcg tgcaaacgtt aagaacaacc aaaactggcg tgattacatg aacaaactcg
     2161 aaggtaaaac tgagggtgaa ttgcaattgt atcaagttta caatgcaatc aacaaacatg
     2221 ctgatcaaga cgctatctac tcaatcgacg taggtaacac tactcaaaca tctactcgtc
     2281 accttcacat gacacctaag aatatgtggc gtacatctcc gctctttgcg acaatgggta
     2341 ttgcccttcc tggtggtatc gctgctaaga aagacaatcc agatcgccaa gtatggaaca
     2401 tcatgggtga tggagcattc aacatgtgct acccagacgt tatcacaaac gttcaatacg
     2461 accttccagt tatcaacctt gtcttctcaa atgctgagta cggcttcatc aagaacaaat
     2521 acgaagatac aaacaaacac ttgtttggtg tagacttcac aaacgctgac tacgctaaaa
     2581 ttgcggaagc tcaaggagct gttggattca cagttgaccg tatcgaagac atcgatgcag
     2641 ttgttgcaga agctgttaaa ttgaacaaag aaggtaaaac tgttgtcatc gatgctcgca
     2701 tcactcaaca ccgtccactt ccagtagaag tacttgaatt ggatccaaaa cttcactcag
     2761 aagaagctat caaagccttc aaggaaaaat acgaagcaga agaactcgta ccattccgtc
     2821 tcttcttgga agaagaagga ttgcaatcac gcgcaattaa ataataa
//