LOCUS BBa_S04052 4498 bp DNA linear 29-Dec-2024 DEFINITION K137004:S03973 ACCESSION BBa_S04052 VERSION BBa_S04052.1 FEATURES Location/Qualifiers CDS 1..1260 /label=BBa_K137004 misc_feature 1273..1276 /label=conserved RBS 1269..1280 /label=BBa_B0034 misc_feature 4310..4310 /label=C->T CDS 1287..4361 /label=lacZ misc_feature 1287..1289 /label=START misc_feature 4359..4361 /label=STOP CDS 1287..4361 /label=BBa_I732005 stem_loop 4381..4424 /label=stem_loop misc_feature 4370..4449 /label=BBa_B0010 terminator 4370..4449 /label=BBa_B0010 stem_loop 4465..4484 /label=T7 TE misc_feature 4491..4491 /label=stop polyA_site 4485..4498 /label=polya misc_feature 4458..4498 /label=BBa_B0012 terminator 4458..4498 /label=BBa_B0012 ORIGIN 1 atgtactatt taaaaaacac aaacttttgg atgttcggtt tattcttttt cttttacttt 61 tttatcatgg gagcctactt cccgtttttc ccgatttggc tacatgacat caaccatatc 121 agcaaaagtg atacgggtat tatttttgcc gctatttctc tgttctcgct attattccaa 181 ccgctgtttg gtctgctttc tgacaaactc gggctgcgca aatacctgct gtggattatt 241 accggcatgt tagtgatgtt tgcgccgttc tttattttta tcttcgggcc actgttacaa 301 tacaacattt tagtaggatc gattgttggt ggtatttatc taggcttttg ttttaacgcc 361 ggtgcgccag cagtagaggc atttattgag aaagtcagcc gtcgcagtaa tttcgaattt 421 ggtcgcgcgc ggatgtttgg ctgtgttggc tgggcgctgt gtgcctcgat tgtcggcatc 481 atgttcacca tcaataatca gtttgttttc tggctgggct ctggctgtgc actcatcctc 541 gccgttttac tctttttcgc caaaacggat gcgccctctt ctcaccacgc cacggttgcc 601 aatgcggtag gtgccaacca ttcggcattt agccttaagc tggcactgga actgttcaga 661 cagccaaaac tgtggttttt gtcactgtat gttattggcg tttcctgcac ctacgatgtt 721 tttgaccaac agtttgctaa tttctttact tcgttctttg ctaccggtga acagggtacg 781 cgggtatttg gctacgtaac gacaatgggc gaattactta acgcctcgat tatgttcttt 841 gcgccactga tcattaatcg catcggtggg aaaaacgccc tgctgctggc tggcactatt 901 atgtctgtac gtattattgg ctcatcgttc gccacctcag cgctggaagt ggttattctg 961 aaaacgctgc atatgtttga agtaccgttc ctgctggtgg gctgctttaa atatattacc 1021 agccagtttg aagtgcgttt ttcagcgacg atttatctgg tctgtttctg cttctttaag 1081 caactggcga tgatttttat gtctgtactg gcgggcaata tgtatgaaag catcggtttc 1141 cagggcgctt atctggtgct gggtctggtg gcgctgggct tcaccttaat ttccgtgttc 1201 acgcttagcg gccccggccc gctttccctg ctgcgtcgtc aggtgaatga agtcgcttaa 1261 tactagagaa agaggagaaa tactagatga ccatgattac ggattcactg gccgtcgttt 1321 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 1381 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 1441 tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg 1501 aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc 1561 agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt acggtcaatc 1621 cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg 1681 aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac tcggcgtttc 1741 atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg ccgtctgaat 1801 ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgct 1861 ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc attttccgtg 1921 acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt gccactcgct 1981 ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc 2041 gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc gccagcggca 2101 ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat cgcgtcacac 2161 tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg 2221 cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc tgcgatgtcg 2281 gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag ccgttgctga 2341 ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga 2401 cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg cgctgttcgc 2461 attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg 2521 atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc 2581 gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc 2641 cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat cacgacgcgc 2701 tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag 2761 ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat gaagaccagc 2821 ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct ggagagacgc 2881 gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta 2941 aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc tgggactggg 3001 tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct tacggcggtg 3061 attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc tttgccgacc 3121 gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc cagttccgtt 3181 tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc gataacgagc 3241 tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa gtgcctctgg 3301 atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag ccggagagcg 3361 ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag 3421 ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt gtgacgctcc 3481 ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt tgcatcgagc 3541 tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag atgtggattg 3601 gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca ccgctggata 3661 acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga 3721 aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca gatacacttg 3781 ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa accttattta 3841 tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc gttgatgttg 3901 aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag ctggcgcagg 3961 tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc gaccgcctta 4021 ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc ccgtacgtct 4081 tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc ccacaccagt 4141 ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg atggaaacca 4201 gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac ggtttccata 4261 tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattt cagctgagcg 4321 ccggtcgcta ccattaccag ttggtctggt gtcaaaaata atactagagc caggcatcaa 4381 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 4441 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //