LOCUS BBa_S04053 9002 bp DNA linear 22-Nov-2024 DEFINITION S04052:S04052 ACCESSION BBa_S04053 VERSION BBa_S04053.1 FEATURES Location/Qualifiers CDS 1..1260 /label=BBa_K137004 misc_feature 1273..1276 /label=conserved RBS 1269..1280 /label=BBa_B0034 misc_feature 4310..4310 /label=C->T CDS 1287..4361 /label=lacZ misc_feature 1287..1289 /label=START misc_feature 4359..4361 /label=STOP CDS 1287..4361 /label=BBa_I732005 stem_loop 4381..4424 /label=stem_loop misc_feature 4370..4449 /label=BBa_B0010 terminator 4370..4449 /label=BBa_B0010 stem_loop 4465..4484 /label=T7 TE misc_feature 4491..4491 /label=stop polyA_site 4485..4498 /label=polya misc_feature 4458..4498 /label=BBa_B0012 terminator 4458..4498 /label=BBa_B0012 CDS 4505..5764 /label=BBa_K137004 misc_feature 5777..5780 /label=conserved RBS 5773..5784 /label=BBa_B0034 misc_feature 8814..8814 /label=C->T CDS 5791..8865 /label=lacZ misc_feature 5791..5793 /label=START misc_feature 8863..8865 /label=STOP CDS 5791..8865 /label=BBa_I732005 stem_loop 8885..8928 /label=stem_loop misc_feature 8874..8953 /label=BBa_B0010 terminator 8874..8953 /label=BBa_B0010 stem_loop 8969..8988 /label=T7 TE misc_feature 8995..8995 /label=stop polyA_site 8989..9002 /label=polya misc_feature 8962..9002 /label=BBa_B0012 terminator 8962..9002 /label=BBa_B0012 ORIGIN 1 atgtactatt taaaaaacac aaacttttgg atgttcggtt tattcttttt cttttacttt 61 tttatcatgg gagcctactt cccgtttttc ccgatttggc tacatgacat caaccatatc 121 agcaaaagtg atacgggtat tatttttgcc gctatttctc tgttctcgct attattccaa 181 ccgctgtttg gtctgctttc tgacaaactc gggctgcgca aatacctgct gtggattatt 241 accggcatgt tagtgatgtt tgcgccgttc tttattttta tcttcgggcc actgttacaa 301 tacaacattt tagtaggatc gattgttggt ggtatttatc taggcttttg ttttaacgcc 361 ggtgcgccag cagtagaggc atttattgag aaagtcagcc gtcgcagtaa tttcgaattt 421 ggtcgcgcgc ggatgtttgg ctgtgttggc tgggcgctgt gtgcctcgat tgtcggcatc 481 atgttcacca tcaataatca gtttgttttc tggctgggct ctggctgtgc actcatcctc 541 gccgttttac tctttttcgc caaaacggat gcgccctctt ctcaccacgc cacggttgcc 601 aatgcggtag gtgccaacca ttcggcattt agccttaagc tggcactgga actgttcaga 661 cagccaaaac tgtggttttt gtcactgtat gttattggcg tttcctgcac ctacgatgtt 721 tttgaccaac agtttgctaa tttctttact tcgttctttg ctaccggtga acagggtacg 781 cgggtatttg gctacgtaac gacaatgggc gaattactta acgcctcgat tatgttcttt 841 gcgccactga tcattaatcg catcggtggg aaaaacgccc tgctgctggc tggcactatt 901 atgtctgtac gtattattgg ctcatcgttc gccacctcag cgctggaagt ggttattctg 961 aaaacgctgc atatgtttga agtaccgttc ctgctggtgg gctgctttaa atatattacc 1021 agccagtttg aagtgcgttt ttcagcgacg atttatctgg tctgtttctg cttctttaag 1081 caactggcga tgatttttat gtctgtactg gcgggcaata tgtatgaaag catcggtttc 1141 cagggcgctt atctggtgct gggtctggtg gcgctgggct tcaccttaat ttccgtgttc 1201 acgcttagcg gccccggccc gctttccctg ctgcgtcgtc aggtgaatga agtcgcttaa 1261 tactagagaa agaggagaaa tactagatga ccatgattac ggattcactg gccgtcgttt 1321 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 1381 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 1441 tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg 1501 aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc 1561 agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt acggtcaatc 1621 cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg 1681 aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac tcggcgtttc 1741 atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg ccgtctgaat 1801 ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgct 1861 ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc attttccgtg 1921 acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt gccactcgct 1981 ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc 2041 gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc gccagcggca 2101 ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat cgcgtcacac 2161 tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg 2221 cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc tgcgatgtcg 2281 gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag ccgttgctga 2341 ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga 2401 cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg cgctgttcgc 2461 attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg 2521 atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc 2581 gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc 2641 cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat cacgacgcgc 2701 tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag 2761 ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat gaagaccagc 2821 ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct ggagagacgc 2881 gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta 2941 aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc tgggactggg 3001 tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct tacggcggtg 3061 attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc tttgccgacc 3121 gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc cagttccgtt 3181 tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc gataacgagc 3241 tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa gtgcctctgg 3301 atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag ccggagagcg 3361 ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag 3421 ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt gtgacgctcc 3481 ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt tgcatcgagc 3541 tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag atgtggattg 3601 gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca ccgctggata 3661 acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga 3721 aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca gatacacttg 3781 ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa accttattta 3841 tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc gttgatgttg 3901 aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag ctggcgcagg 3961 tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc gaccgcctta 4021 ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc ccgtacgtct 4081 tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc ccacaccagt 4141 ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg atggaaacca 4201 gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac ggtttccata 4261 tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattt cagctgagcg 4321 ccggtcgcta ccattaccag ttggtctggt gtcaaaaata atactagagc caggcatcaa 4381 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 4441 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata 4501 ctagatgtac tatttaaaaa acacaaactt ttggatgttc ggtttattct ttttctttta 4561 cttttttatc atgggagcct acttcccgtt tttcccgatt tggctacatg acatcaacca 4621 tatcagcaaa agtgatacgg gtattatttt tgccgctatt tctctgttct cgctattatt 4681 ccaaccgctg tttggtctgc tttctgacaa actcgggctg cgcaaatacc tgctgtggat 4741 tattaccggc atgttagtga tgtttgcgcc gttctttatt tttatcttcg ggccactgtt 4801 acaatacaac attttagtag gatcgattgt tggtggtatt tatctaggct tttgttttaa 4861 cgccggtgcg ccagcagtag aggcatttat tgagaaagtc agccgtcgca gtaatttcga 4921 atttggtcgc gcgcggatgt ttggctgtgt tggctgggcg ctgtgtgcct cgattgtcgg 4981 catcatgttc accatcaata atcagtttgt tttctggctg ggctctggct gtgcactcat 5041 cctcgccgtt ttactctttt tcgccaaaac ggatgcgccc tcttctcacc acgccacggt 5101 tgccaatgcg gtaggtgcca accattcggc atttagcctt aagctggcac tggaactgtt 5161 cagacagcca aaactgtggt ttttgtcact gtatgttatt ggcgtttcct gcacctacga 5221 tgtttttgac caacagtttg ctaatttctt tacttcgttc tttgctaccg gtgaacaggg 5281 tacgcgggta tttggctacg taacgacaat gggcgaatta cttaacgcct cgattatgtt 5341 ctttgcgcca ctgatcatta atcgcatcgg tgggaaaaac gccctgctgc tggctggcac 5401 tattatgtct gtacgtatta ttggctcatc gttcgccacc tcagcgctgg aagtggttat 5461 tctgaaaacg ctgcatatgt ttgaagtacc gttcctgctg gtgggctgct ttaaatatat 5521 taccagccag tttgaagtgc gtttttcagc gacgatttat ctggtctgtt tctgcttctt 5581 taagcaactg gcgatgattt ttatgtctgt actggcgggc aatatgtatg aaagcatcgg 5641 tttccagggc gcttatctgg tgctgggtct ggtggcgctg ggcttcacct taatttccgt 5701 gttcacgctt agcggccccg gcccgctttc cctgctgcgt cgtcaggtga atgaagtcgc 5761 ttaatactag agaaagagga gaaatactag atgaccatga ttacggattc actggccgtc 5821 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 5881 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 5941 cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg 6001 ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac 6061 tggcagatgc acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc 6121 aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt 6181 gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg 6241 tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct 6301 gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg 6361 cgctggagtg acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc 6421 cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact 6481 cgctttaatg atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag 6541 ttgcgtgact acctacgggt aacagtttct ttatggcagg gtgaaacgca ggtcgccagc 6601 ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc 6661 acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat 6721 cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat 6781 gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg 6841 ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag 6901 cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt 6961 tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg 7021 gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat 7081 ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat 7141 cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc taatcacgac 7201 gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc 7261 ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac 7321 cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag 7381 acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc 7441 gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt cgtctgggac 7501 tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc 7561 ggtgattttg gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc 7621 gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc 7681 cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac 7741 gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct 7801 ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag 7861 agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca 7921 gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg 7981 ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc 8041 gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg 8101 attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg 8161 gataacgaca ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc 8221 tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca 8281 cttgctgatg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta 8341 tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat 8401 gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg 8461 caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc 8521 cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac 8581 gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac 8641 cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa 8701 accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc 8761 catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga atttcagctg 8821 agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataatacta gagccaggca 8881 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 8941 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 9001 ta //