LOCUS BBa_S04054 4515 bp DNA linear 15-May-2024 DEFINITION S04022 + S03973 ACCESSION BBa_S04054 VERSION BBa_S04054.1 FEATURES Location/Qualifiers RBS 1..11 /label=RBS-4\Weaker RBS 7..10 /label=RBS misc_feature 1..11 /label=BBa_B0033 RBS 1..11 /label=BBa_B0033 CDS 18..1277 /label=BBa_K137004 misc_feature 1290..1293 /label=conserved RBS 1286..1297 /label=BBa_B0034 misc_feature 4327..4327 /label=C->T CDS 1304..4378 /label=lacZ misc_feature 1304..1306 /label=START misc_feature 4376..4378 /label=STOP CDS 1304..4378 /label=BBa_I732005 stem_loop 4398..4441 /label=stem_loop misc_feature 4387..4466 /label=BBa_B0010 terminator 4387..4466 /label=BBa_B0010 stem_loop 4482..4501 /label=T7 TE misc_feature 4508..4508 /label=stop polyA_site 4502..4515 /label=polya misc_feature 4475..4515 /label=BBa_B0012 terminator 4475..4515 /label=BBa_B0012 ORIGIN 1 tcacacagga ctactagatg tactatttaa aaaacacaaa cttttggatg ttcggtttat 61 tctttttctt ttactttttt atcatgggag cctacttccc gtttttcccg atttggctac 121 atgacatcaa ccatatcagc aaaagtgata cgggtattat ttttgccgct atttctctgt 181 tctcgctatt attccaaccg ctgtttggtc tgctttctga caaactcggg ctgcgcaaat 241 acctgctgtg gattattacc ggcatgttag tgatgtttgc gccgttcttt atttttatct 301 tcgggccact gttacaatac aacattttag taggatcgat tgttggtggt atttatctag 361 gcttttgttt taacgccggt gcgccagcag tagaggcatt tattgagaaa gtcagccgtc 421 gcagtaattt cgaatttggt cgcgcgcgga tgtttggctg tgttggctgg gcgctgtgtg 481 cctcgattgt cggcatcatg ttcaccatca ataatcagtt tgttttctgg ctgggctctg 541 gctgtgcact catcctcgcc gttttactct ttttcgccaa aacggatgcg ccctcttctc 601 accacgccac ggttgccaat gcggtaggtg ccaaccattc ggcatttagc cttaagctgg 661 cactggaact gttcagacag ccaaaactgt ggtttttgtc actgtatgtt attggcgttt 721 cctgcaccta cgatgttttt gaccaacagt ttgctaattt ctttacttcg ttctttgcta 781 ccggtgaaca gggtacgcgg gtatttggct acgtaacgac aatgggcgaa ttacttaacg 841 cctcgattat gttctttgcg ccactgatca ttaatcgcat cggtgggaaa aacgccctgc 901 tgctggctgg cactattatg tctgtacgta ttattggctc atcgttcgcc acctcagcgc 961 tggaagtggt tattctgaaa acgctgcata tgtttgaagt accgttcctg ctggtgggct 1021 gctttaaata tattaccagc cagtttgaag tgcgtttttc agcgacgatt tatctggtct 1081 gtttctgctt ctttaagcaa ctggcgatga tttttatgtc tgtactggcg ggcaatatgt 1141 atgaaagcat cggtttccag ggcgcttatc tggtgctggg tctggtggcg ctgggcttca 1201 ccttaatttc cgtgttcacg cttagcggcc ccggcccgct ttccctgctg cgtcgtcagg 1261 tgaatgaagt cgcttaatac tagagaaaga ggagaaatac tagatgacca tgattacgga 1321 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 1381 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 1441 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc 1501 accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt 1561 cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta 1621 tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct 1681 cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg 1741 cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag 1801 tcgtttgccg tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc 1861 ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa gatcaggata tgtggcggat 1921 gagcggcatt ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt 1981 ccatgttgcc actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca 2041 gatgtgcggc gagttgcgtg actacctacg ggtaacagtt tctttatggc agggtgaaac 2101 gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta 2161 tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat 2221 cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc tgattgaagc 2281 agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa 2341 cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag catcatcctc tgcatggtca 2401 ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa 2461 cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgcta 2521 cggcctgtat gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg 2581 tctgaccgat gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca 2641 gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgaat caggccacgg 2701 cgctaatcac gacgcgctgt atcgctggat caaatctgtc gatccttccc gcccggtgca 2761 gtatgaaggc ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg 2821 cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc 2881 gctacctgga gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag 2941 tcttggcggt ttcgctaaat actggcaggc gtttcgtcag tatccccgtt tacagggcgg 3001 cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaacg gcaacccgtg 3061 gtcggcttac ggcggtgatt ttggcgatac gccgaacgat cgccagttct gtatgaacgg 3121 tctggtcttt gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac accagcagca 3181 gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg 3241 tcatagcgat aacgagctcc tgcactggat ggtggcgctg gatggtaagc cgctggcaag 3301 cggtgaagtg cctctggatg tcgctccaca aggtaaacag ttgattgaac tgcctgaact 3361 accgcagccg gagagcgccg ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc 3421 gaccgcatgg tcagaagccg ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa 3481 cctcagtgtg acgctccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat 3541 ggatttttgc atcgagctgg gtaataagcg ttggcaattt aaccgccagt caggctttct 3601 ttcacagatg tggattggcg ataaaaaaca actgctgacg ccgctgcgcg atcagttcac 3661 ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc 3721 ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg 3781 cacggcagat acacttgctg atgcggtgct gattacgacc gctcacgcgt ggcagcatca 3841 ggggaaaacc ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc 3901 gattaccgtt gatgttgaag tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa 3961 ctgccagctg gcgcaggtag cagagcgggt aaactggctc ggattagggc cgcaagaaaa 4021 ctatcccgac cgccttactg ccgcctgttt tgaccgctgg gatctgccat tgtcagacat 4081 gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa 4141 ttatggccca caccagtggc gcggcgactt ccagttcaac atcagccgct acagtcaaca 4201 gcaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa 4261 tatcgacggt ttccatatgg ggattggtgg cgacgactcc tggagcccgt cagtatcggc 4321 ggaatttcag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaataata 4381 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 4441 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 4501 ctttctgcgt ttata //