LOCUS BBa_S04055 4558 bp DNA linear 14-May-2024 DEFINITION Synthetic lacYZ operon ACCESSION BBa_S04055 VERSION BBa_S04055.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 RBS 44..54 /label=RBS-4\Weaker RBS 50..53 /label=RBS misc_feature 44..54 /label=BBa_B0033 RBS 44..54 /label=BBa_B0033 CDS 61..1320 /label=BBa_K137004 misc_feature 1333..1336 /label=conserved RBS 1329..1340 /label=BBa_B0034 misc_feature 4370..4370 /label=C->T CDS 1347..4421 /label=lacZ misc_feature 1347..1349 /label=START misc_feature 4419..4421 /label=STOP CDS 1347..4421 /label=BBa_I732005 stem_loop 4441..4484 /label=stem_loop misc_feature 4430..4509 /label=BBa_B0010 terminator 4430..4509 /label=BBa_B0010 stem_loop 4525..4544 /label=T7 TE misc_feature 4551..4551 /label=stop polyA_site 4545..4558 /label=polya misc_feature 4518..4558 /label=BBa_B0012 terminator 4518..4558 /label=BBa_B0012 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagtcacaca ggactactag 61 atgtactatt taaaaaacac aaacttttgg atgttcggtt tattcttttt cttttacttt 121 tttatcatgg gagcctactt cccgtttttc ccgatttggc tacatgacat caaccatatc 181 agcaaaagtg atacgggtat tatttttgcc gctatttctc tgttctcgct attattccaa 241 ccgctgtttg gtctgctttc tgacaaactc gggctgcgca aatacctgct gtggattatt 301 accggcatgt tagtgatgtt tgcgccgttc tttattttta tcttcgggcc actgttacaa 361 tacaacattt tagtaggatc gattgttggt ggtatttatc taggcttttg ttttaacgcc 421 ggtgcgccag cagtagaggc atttattgag aaagtcagcc gtcgcagtaa tttcgaattt 481 ggtcgcgcgc ggatgtttgg ctgtgttggc tgggcgctgt gtgcctcgat tgtcggcatc 541 atgttcacca tcaataatca gtttgttttc tggctgggct ctggctgtgc actcatcctc 601 gccgttttac tctttttcgc caaaacggat gcgccctctt ctcaccacgc cacggttgcc 661 aatgcggtag gtgccaacca ttcggcattt agccttaagc tggcactgga actgttcaga 721 cagccaaaac tgtggttttt gtcactgtat gttattggcg tttcctgcac ctacgatgtt 781 tttgaccaac agtttgctaa tttctttact tcgttctttg ctaccggtga acagggtacg 841 cgggtatttg gctacgtaac gacaatgggc gaattactta acgcctcgat tatgttcttt 901 gcgccactga tcattaatcg catcggtggg aaaaacgccc tgctgctggc tggcactatt 961 atgtctgtac gtattattgg ctcatcgttc gccacctcag cgctggaagt ggttattctg 1021 aaaacgctgc atatgtttga agtaccgttc ctgctggtgg gctgctttaa atatattacc 1081 agccagtttg aagtgcgttt ttcagcgacg atttatctgg tctgtttctg cttctttaag 1141 caactggcga tgatttttat gtctgtactg gcgggcaata tgtatgaaag catcggtttc 1201 cagggcgctt atctggtgct gggtctggtg gcgctgggct tcaccttaat ttccgtgttc 1261 acgcttagcg gccccggccc gctttccctg ctgcgtcgtc aggtgaatga agtcgcttaa 1321 tactagagaa agaggagaaa tactagatga ccatgattac ggattcactg gccgtcgttt 1381 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 1441 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 1501 tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg 1561 aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc 1621 agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt acggtcaatc 1681 cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg 1741 aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac tcggcgtttc 1801 atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg ccgtctgaat 1861 ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgct 1921 ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc attttccgtg 1981 acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt gccactcgct 2041 ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc 2101 gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc gccagcggca 2161 ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat cgcgtcacac 2221 tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg 2281 cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc tgcgatgtcg 2341 gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag ccgttgctga 2401 ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga 2461 cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg cgctgttcgc 2521 attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg 2581 atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc 2641 gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc 2701 cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat cacgacgcgc 2761 tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag 2821 ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat gaagaccagc 2881 ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct ggagagacgc 2941 gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta 3001 aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc tgggactggg 3061 tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct tacggcggtg 3121 attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc tttgccgacc 3181 gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc cagttccgtt 3241 tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc gataacgagc 3301 tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa gtgcctctgg 3361 atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag ccggagagcg 3421 ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag 3481 ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt gtgacgctcc 3541 ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt tgcatcgagc 3601 tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag atgtggattg 3661 gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca ccgctggata 3721 acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga 3781 aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca gatacacttg 3841 ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa accttattta 3901 tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc gttgatgttg 3961 aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag ctggcgcagg 4021 tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc gaccgcctta 4081 ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc ccgtacgtct 4141 tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc ccacaccagt 4201 ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg atggaaacca 4261 gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac ggtttccata 4321 tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattt cagctgagcg 4381 ccggtcgcta ccattaccag ttggtctggt gtcaaaaata atactagagc caggcatcaa 4441 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 4501 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //