##gff-version 3
##sequence-region BBa_S04055 1 4558
BBa_S04055	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_S04055	.	terminator	4518	4558	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S04055	.	stop_codon	4551	4551	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04055	.	polyA_site	4545	4558	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04055	.	stem_loop	4525	4544	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04055	.	engineered_region	4518	4558	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04055	.	ribosome_entry_site	1329	1340	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S04055	.	conserved_region	1333	1336	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04055	.	CDS	61	1320	.	+	0	ID=BBa_K137004;Name=BBa_K137004
BBa_S04055	.	ribosome_entry_site	44	54	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_S04055	.	ribosome_entry_site	44	54	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_S04055	.	engineered_region	44	54	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_S04055	.	ribosome_entry_site	50	53	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_S04055	.	terminator	4430	4509	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S04055	.	engineered_region	4430	4509	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04055	.	stem_loop	4441	4484	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04055	.	CDS	1347	4421	.	+	0	ID=BBa_I732005;Name=BBa_I732005
BBa_S04055	.	point_mutation	4370	4370	.	+	0	ID=annotation1936869;Name=C->T;Parent=BBa_I732005
BBa_S04055	.	stop_codon	4419	4421	.	+	0	ID=annotation1936872;Name=STOP;Parent=BBa_I732005
BBa_S04055	.	start_codon	1347	1349	.	+	0	ID=annotation1936871;Name=START;Parent=BBa_I732005
BBa_S04055	.	CDS	1347	4421	.	+	0	ID=annotation1936870;Name=lacZ;Parent=BBa_I732005
>BBa_S04055
ttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggactactagatgtactatttaaaaaaca
caaacttttggatgttcggtttattctttttcttttacttttttatcatgggagcctacttcccgtttttcccgatttg
gctacatgacatcaaccatatcagcaaaagtgatacgggtattatttttgccgctatttctctgttctcgctattattc
caaccgctgtttggtctgctttctgacaaactcgggctgcgcaaatacctgctgtggattattaccggcatgttagtga
tgtttgcgccgttctttatttttatcttcgggccactgttacaatacaacattttagtaggatcgattgttggtggtat
ttatctaggcttttgttttaacgccggtgcgccagcagtagaggcatttattgagaaagtcagccgtcgcagtaatttc
gaatttggtcgcgcgcggatgtttggctgtgttggctgggcgctgtgtgcctcgattgtcggcatcatgttcaccatca
ataatcagtttgttttctggctgggctctggctgtgcactcatcctcgccgttttactctttttcgccaaaacggatgc
gccctcttctcaccacgccacggttgccaatgcggtaggtgccaaccattcggcatttagccttaagctggcactggaa
ctgttcagacagccaaaactgtggtttttgtcactgtatgttattggcgtttcctgcacctacgatgtttttgaccaac
agtttgctaatttctttacttcgttctttgctaccggtgaacagggtacgcgggtatttggctacgtaacgacaatggg
cgaattacttaacgcctcgattatgttctttgcgccactgatcattaatcgcatcggtgggaaaaacgccctgctgctg
gctggcactattatgtctgtacgtattattggctcatcgttcgccacctcagcgctggaagtggttattctgaaaacgc
tgcatatgtttgaagtaccgttcctgctggtgggctgctttaaatatattaccagccagtttgaagtgcgtttttcagc
gacgatttatctggtctgtttctgcttctttaagcaactggcgatgatttttatgtctgtactggcgggcaatatgtat
gaaagcatcggtttccagggcgcttatctggtgctgggtctggtggcgctgggcttcaccttaatttccgtgttcacgc
ttagcggccccggcccgctttccctgctgcgtcgtcaggtgaatgaagtcgcttaatactagagaaagaggagaaatac
tagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaactta
atcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagtt
gcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgat
cttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtga
cctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttga
tgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacggg
cgctgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccggagaaaacc
gcctcgcggtgatggtgctgcgctggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcatttt
ccgtgacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcgctttaatgatgatttc
agccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggc
agggtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcg
cgtcacactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaa
ctgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtc
tgctgctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcat
ggatgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccg
aaccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggca
tggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcg
cgatcgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccacggcgctaatcacgacgcgctgtat
cgctggatcaaatctgtcgatccttcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccgatatta
tttgcccgatgtacgcgcgcgtggatgaagaccagcccttcccggctgtgccgaaatggtccatcaaaaaatggctttc
gctacctggagagacgcgcccgctgatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaa
tactggcaggcgtttcgtcagtatccccgtttacagggcggcttcgtctgggactgggtggatcagtcgctgattaaat
atgatgaaaacggcaacccgtggtcggcttacggcggtgattttggcgatacgccgaacgatcgccagttctgtatgaa
cggtctggtctttgccgaccgcacgccgcatccagcgctgacggaagcaaaacaccagcagcagtttttccagttccgt
ttatccgggcaaaccatcgaagtgaccagcgaatacctgttccgtcatagcgataacgagctcctgcactggatggtgg
cgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcc
tgaactaccgcagccggagagcgccgggcaactctggctcacagtacgcgtagtgcaaccgaacgcgaccgcatggtca
gaagccgggcacatcagcgcctggcagcagtggcgtctggcggaaaacctcagtgtgacgctccccgccgcgtcccacg
ccatcccgcatctgaccaccagcgaaatggatttttgcatcgagctgggtaataagcgttggcaatttaaccgccagtc
aggctttctttcacagatgtggattggcgataaaaaacaactgctgacgccgctgcgcgatcagttcacccgtgcaccg
ctggataacgacattggcgtaagtgaagcgacccgcattgaccctaacgcctgggtcgaacgctggaaggcggcgggcc
attaccaggccgaagcagcgttgttgcagtgcacggcagatacacttgctgatgcggtgctgattacgaccgctcacgc
gtggcagcatcaggggaaaaccttatttatcagccggaaaacctaccggattgatggtagtggtcaaatggcgattacc
gttgatgttgaagtggcgagcgatacaccgcatccggcgcggattggcctgaactgccagctggcgcaggtagcagagc
gggtaaactggctcggattagggccgcaagaaaactatcccgaccgccttactgccgcctgttttgaccgctgggatct
gccattgtcagacatgtataccccgtacgtcttcccgagcgaaaacggtctgcgctgcgggacgcgcgaattgaattat
ggcccacaccagtggcgcggcgacttccagttcaacatcagccgctacagtcaacagcaactgatggaaaccagccatc
gccatctgctgcacgcggaagaaggcacatggctgaatatcgacggtttccatatggggattggtggcgacgactcctg
gagcccgtcagtatcggcggaatttcagctgagcgccggtcgctaccattaccagttggtctggtgtcaaaaataatac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata