LOCUS BBa_S04056 5338 bp DNA linear 07-Dec-2025 DEFINITION pLux/cI+pLac/Mnt+LuxR+LacI ACCESSION BBa_S04056 VERSION BBa_S04056.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=Lux Box promoter 20..25 /label=Lux -35 misc_feature 26..43 /label=cI OR2 promoter 44..49 /label=Lux -10 promoter 1..57 /label=BBa_K091107 misc_feature 70..73 /label=conserved RBS 66..77 /label=BBa_B0034 CDS 84..692 /label=lasI CDS 84..692 /label=BBa_C0178 misc_feature 705..708 /label=conserved RBS 701..712 /label=BBa_B0034 CDS 719..967 /label=mnt misc_feature 813..815 /label=T misc_feature 968..1000 /label=LVA misc_feature 1007..1031 /label=Help:Barcodes CDS 719..1006 /label=BBa_C0072 stem_loop 1051..1094 /label=stem_loop misc_feature 1040..1119 /label=BBa_B0010 terminator 1040..1119 /label=BBa_B0010 stem_loop 1135..1154 /label=T7 TE misc_feature 1161..1161 /label=stop polyA_site 1155..1168 /label=polya misc_feature 1128..1168 /label=BBa_B0012 terminator 1128..1168 /label=BBa_B0012 promoter 1223..1228 /label=-10 misc_feature 1229..1263 /label=LacI misc_feature 1177..1228 /label=Mnt promoter 1177..1263 /label=BBa_K091104 misc_feature 1276..1279 /label=conserved RBS 1272..1283 /label=BBa_B0034 CDS 1290..1898 /label=lasI CDS 1290..1898 /label=BBa_C0178 misc_feature 1911..1914 /label=conserved RBS 1907..1918 /label=BBa_B0034 CDS 1928..2635 /label=cI lambda misc_feature 2636..2668 /label=LVA misc_feature 2675..2699 /label=Help:Barcodes CDS 1925..2674 /label=BBa_C0051 stem_loop 2715..2734 /label=T7 TE misc_feature 2741..2741 /label=stop polyA_site 2735..2748 /label=polya misc_feature 2708..2748 /label=BBa_B0012 terminator 2708..2748 /label=BBa_B0012 stem_loop 2769..2791 /label=stem_loop misc_feature 2757..2802 /label=BBa_B0011 terminator 2757..2802 /label=BBa_B0011 promoter 2811..2940 /label=BBa_I13453 misc_feature 2953..2956 /label=conserved RBS 2949..2960 /label=BBa_B0034 misc_feature 4056..4094 /label=LVA CDS 2967..4094 /label=lacI-LVA misc_feature 2967..4094 /label=BBa_C0012 misc_feature 4095..4119 /label=Help:Barcodes CDS 2967..4094 /label=BBa_C0012 stem_loop 4139..4182 /label=stem_loop misc_feature 4128..4207 /label=BBa_B0010 terminator 4128..4207 /label=BBa_B0010 stem_loop 4223..4242 /label=T7 TE misc_feature 4249..4249 /label=stop polyA_site 4243..4256 /label=polya misc_feature 4216..4256 /label=BBa_B0012 terminator 4216..4256 /label=BBa_B0012 promoter 4265..4394 /label=BBa_I13453 misc_feature 4407..4410 /label=conserved RBS 4403..4414 /label=BBa_B0034 misc_feature 4421..4422 /label=prefix misc_feature 4594..4594 /label=T misc_feature 4912..4912 /label=A CDS 4421..5170 /label=luxR misc_feature 4421..5176 /label=BBa_C0062 misc_feature 5177..5201 /label=Help:Barcodes CDS 4421..5176 /label=BBa_C0062 stem_loop 5221..5264 /label=stem_loop misc_feature 5210..5289 /label=BBa_B0010 terminator 5210..5289 /label=BBa_B0010 stem_loop 5305..5324 /label=T7 TE misc_feature 5331..5331 /label=stop polyA_site 5325..5338 /label=polya misc_feature 5298..5338 /label=BBa_B0012 terminator 5298..5338 /label=BBa_B0012 ORIGIN 1 acctgtagga tcgtacaggt ttacgtaaca ccgtgcgtgt tgatatagtc gaataaatac 61 tagagaaaga ggagaaatac tagatgatcg ttcagatcgg tcgtcgtgaa gagttcgaca 121 aaaaactgct gggtgaaatg cacaaactgc gtgctcaggt tttcaaagaa cgtaaaggtt 181 gggacgtttc cgttatcgac gaaatggaaa tcgacggtta cgacgctctg tccccgtact 241 acatgctgat ccaggaagac accccggaag ctcaggtttt cggttgctgg cgtatcttcg 301 acaccaccgg tccgtacatg ctgaaaaaca ccttcccgga actgctgcac ggtaaagaag 361 ctccgtgctc cccgcacatc tgggaactgt cccgtttcgc tatcaactcc ggtcagaaag 421 gttccctggg tttctccgac tgcaccctgg aagctatgcg tgctctggct cgttactcct 481 tgcagaacga catccagacc ctggttaccg ttaccaccgt tggtgttgaa aaaatgatga 541 tccgtgctgg tctggacgtt tcccgtttcg gtccgcacct gaaaatcggt atcgaacgtg 601 ctgttgctct gcgtatcgaa ctgaacgcta aaacccagat cgctctgtac ggtggtgttc 661 tggttgaaca gcgtctggct gtttcctaat aatactagag aaagaggaga aatactagat 721 ggcccgggat gatcctcact tcaattttcg tatgccaatg gaagtaagag agaaattgaa 781 atttagagca gaggcaaacg gacggagcat gaactctgag cttttgcaaa tcgtacaaga 841 tgccctaagc aaaccgtcac cagtcactgg gtaccgcaat gatgcggaac gactcgccga 901 tgagcagagc gagttagtga agaagatggt cttcgataca ctgaaggatc tttataaaaa 961 aaccaccgct gcaaacgacg aaaactacgc tttagtagct taataaccct gatagtgcta 1021 gtgtagatcc ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 1081 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 1141 ccttcgggtg ggcctttctg cgtttatata ctagagctcg aggtgagtgc acagtactag 1201 gtccacggtg acctagatct cctatagttg tgtggaattg tgagcggata acaatttcac 1261 acatactaga gaaagaggag aaatactaga tgatcgttca gatcggtcgt cgtgaagagt 1321 tcgacaaaaa actgctgggt gaaatgcaca aactgcgtgc tcaggttttc aaagaacgta 1381 aaggttggga cgtttccgtt atcgacgaaa tggaaatcga cggttacgac gctctgtccc 1441 cgtactacat gctgatccag gaagacaccc cggaagctca ggttttcggt tgctggcgta 1501 tcttcgacac caccggtccg tacatgctga aaaacacctt cccggaactg ctgcacggta 1561 aagaagctcc gtgctccccg cacatctggg aactgtcccg tttcgctatc aactccggtc 1621 agaaaggttc cctgggtttc tccgactgca ccctggaagc tatgcgtgct ctggctcgtt 1681 actccttgca gaacgacatc cagaccctgg ttaccgttac caccgttggt gttgaaaaaa 1741 tgatgatccg tgctggtctg gacgtttccc gtttcggtcc gcacctgaaa atcggtatcg 1801 aacgtgctgt tgctctgcgt atcgaactga acgctaaaac ccagatcgct ctgtacggtg 1861 gtgttctggt tgaacagcgt ctggctgttt cctaataata ctagagaaag aggagaaata 1921 ctagatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg cacgtcgcct 1981 taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat ctgtcgcaga 2041 caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca atgcattaaa 2101 tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag aatttagccc 2161 ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc cgtcacttag 2221 aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct cacctgagct 2281 tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa aagccagtga 2341 ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc 2401 aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg agccaggtga 2461 tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga tcagggatag 2521 cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat gcaatgagag 2581 ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt ttggcgctgc 2641 aaacgacgaa aactacgctt tagtagctta ataacgctga tagtgctagt gtagatcgct 2701 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag 2761 aatataaaaa gccagattat taatccggct tttttattat tttactagag acattgatta 2821 tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat tagcggatcc 2881 tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt ttgggctagc 2941 tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg ttatacgatg 3001 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc 3061 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc 3121 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct 3181 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc 3241 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag 3301 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg 3361 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg 3421 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac 3481 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat 3541 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc 3601 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa 3661 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga 3721 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct 3781 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca 3841 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc 3901 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca 3961 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 4021 tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa aactacgctt 4081 tagtagctta ataactctga tagtgctagt gtagatctct actagagcca ggcatcaaat 4141 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 4201 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 4261 agagacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata 4321 agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc tccataccgt 4381 ttttttgggc tagctactag agaaagagga gaaatactag atgaaaaaca taaatgccga 4441 cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg atattaatca 4501 atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg cgatcattta 4561 tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta aaaaatggag 4621 gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt attctaactc 4681 caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc 4741 aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt tccctattca 4801 tacggctaac aatggcttcg gaatgcttag ttttgcacat tcagaaaaag acaactatat 4861 agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt ctctagttga 4921 taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa ccaaaagaga 4981 aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt caaaaatatt 5041 aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga aactcaatac 5101 aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg attgcccata 5161 ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa 5221 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 5281 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //