##gff-version 3
##sequence-region BBa_S04057 1 3797
BBa_S04057	.	engineered_region	2876	3797	.	+	0	ID=BBa_S03948;Name=BBa_S03948
BBa_S04057	.	engineered_region	2876	3777	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400;Parent=BBa_S03948
BBa_S04057	.	ribosome_entry_site	2876	2887	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_S04057	.	conserved_region	2880	2883	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04057	.	CDS	2894	3553	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_S04057	.	engineered_tag	3554	3578	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S04057	.	tag	3514	3547	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S04057	.	CDS	2897	3513	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S04057	.	terminator	3675	3715	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_S04057	.	engineered_region	3675	3715	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04057	.	stop_codon	3708	3708	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04057	.	stem_loop	3682	3701	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04057	.	polyA_site	3702	3715	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04057	.	promoter	3724	3777	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_S04057	.	promoter	3743	3748	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S04057	.	non_covalent_binding_site	3749	3767	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S04057	.	engineered_region	3724	3777	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S04057	.	non_covalent_binding_site	3724	3742	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S04057	.	promoter	3766	3771	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S04057	.	terminator	3587	3666	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_S04057	.	stem_loop	3598	3641	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04057	.	engineered_region	3587	3666	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04057	.	ribosome_entry_site	3786	3797	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03948
BBa_S04057	.	conserved_region	3790	3793	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04057	.	engineered_region	1	2867	.	+	0	ID=BBa_S04051;Name=BBa_S04051
BBa_S04057	.	CDS	1089	2867	.	+	0	ID=BBa_K137069;Name=BBa_K137069;Parent=BBa_S04051
BBa_S04057	.	sequence_alteration	1115	1115	.	+	0	ID=annotation1969567;Name=T->G;Parent=BBa_K137069
BBa_S04057	.	engineered_region	1	1082	.	+	0	ID=BBa_S03975;Name=BBa_S03975;Parent=BBa_S04051
BBa_S04057	.	engineered_region	1	1062	.	+	0	ID=BBa_F2622;Name=BBa_F2622;Parent=BBa_S03975
BBa_S04057	.	terminator	871	950	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2622
BBa_S04057	.	stem_loop	882	925	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04057	.	engineered_region	871	950	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04057	.	CDS	82	837	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2622
BBa_S04057	.	CDS	82	831	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04057	.	engineered_region	82	837	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04057	.	engineered_tag	838	862	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04057	.	point_mutation	255	255	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04057	.	engineered_region	82	83	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04057	.	point_mutation	573	573	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04057	.	promoter	1008	1062	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2622
BBa_S04057	.	non_covalent_binding_site	1008	1027	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_S04057	.	promoter	1049	1054	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_S04057	.	engineered_region	1008	1062	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_S04057	.	start_codon	1060	1060	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_S04057	.	promoter	1027	1032	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_S04057	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_F2622
BBa_S04057	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_S04057	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_S04057	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_S04057	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_S04057	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_S04057	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2622
BBa_S04057	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04057	.	terminator	959	999	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2622
BBa_S04057	.	engineered_region	959	999	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04057	.	stop_codon	992	992	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04057	.	stem_loop	966	985	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04057	.	polyA_site	986	999	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04057	.	ribosome_entry_site	1071	1082	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03975
BBa_S04057	.	conserved_region	1075	1078	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S04057
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaa
tcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtt
aaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatg
atcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaa
atctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaat
ggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaaca
taccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaac
caaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagt
gagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaag
caattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggt
ttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgactcaagggaaaatt
actgcatcggcagcaatgcttaacgtattgaaaacatggggcgtagatacaatctacggtatcccatcaggaacactca
gctcattgatggacgctttggctgaagacaaagatatccgcttcttacaagttcgccacgaagagacaggtgctcttgc
agcggttatgcaagctaaattcggcggctcaatcggggttgcagttggttcaggtggtccaggtgcgactcacttgatt
aacggtgtttacgatgcagctatggatgacactccattcctagcgatccttggatcacgtccagttaacgaattgaaca
tggatgctttccaagagcttaaccaaaacccaatgtacaacggtatcgctgtttacaacaaacgtgtagcttacgctga
gcaattgccaaaagtaattgacgaagcctgccgtgctgcaatttctaaaaaaggtccagctgttgttgaaattccagta
aacttcggtttccaagaaatcgacgaaaactcatactacggttcaggttcatacgaacgctcattcatcgctcctgctt
tgaacgaagttgaaatcgacaaagctgttgaaatcttgaacaatgctgaacgcccagttatctatgctggatttggtgg
tgttaaagctggtgaagtgattactgaattgtcacgtaaaatcaaagcaccaatcatcacaactggtaaaaactttgaa
gctttcgaatggaactatgaaggtttgacaggttctgcttaccgtgttggttggaaaccagccaacgaagtggtctttg
aagcagacacagttcttttccttggttcaaacttcgcatttgctgaagtttacgaagcattcaagaacactgaaaaatt
catacaagtcgatatcgacccttacaaacttggtaaacgtcatgcccttgacgcttcaatccttggtgatgctggtcaa
gcagctaaagctatccttgacaaagtaaacccagttgaatcaactccatggtggcgtgcaaacgttaagaacaaccaaa
actggcgtgattacatgaacaaactcgaaggtaaaactgagggtgaattgcaattgtatcaagtttacaatgcaatcaa
caaacatgctgatcaagacgctatctactcaatcgacgtaggtaacactactcaaacatctactcgtcaccttcacatg
acacctaagaatatgtggcgtacatctccgctctttgcgacaatgggtattgcccttcctggtggtatcgctgctaaga
aagacaatccagatcgccaagtatggaacatcatgggtgatggagcattcaacatgtgctacccagacgttatcacaaa
cgttcaatacgaccttccagttatcaaccttgtcttctcaaatgctgagtacggcttcatcaagaacaaatacgaagat
acaaacaaacacttgtttggtgtagacttcacaaacgctgactacgctaaaattgcggaagctcaaggagctgttggat
tcacagttgaccgtatcgaagacatcgatgcagttgttgcagaagctgttaaattgaacaaagaaggtaaaactgttgt
catcgatgctcgcatcactcaacaccgtccacttccagtagaagtacttgaattggatccaaaacttcactcagaagaa
gctatcaaagccttcaaggaaaaatacgaagcagaagaactcgtaccattccgtctcttcttggaagaagaaggattgc
aatcacgcgcaattaaataataatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgatt
aacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtag
agcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggca
ccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgct
ttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatc
aattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttacttt
aggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgcca
ttattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatat
gcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataaca
ctgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagagg
agaaa