LOCUS       BBa_S04058              1238 bp    DNA     linear       02-May-2025
DEFINITION  RBS-LacI12-TT
ACCESSION   BBa_S04058
VERSION     BBa_S04058.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    25..27
                     /label=Proline->Tyrosine
     CDS             19..1101
                     /label=BBa_K091122
     stem_loop       1121..1164
                     /label=stem_loop
     misc_feature    1110..1189
                     /label=BBa_B0010
     terminator      1110..1189
                     /label=BBa_B0010
     stem_loop       1205..1224
                     /label=T7 TE
     misc_feature    1231..1231
                     /label=stop
     polyA_site      1225..1238
                     /label=polya
     misc_feature    1198..1238
                     /label=BBa_B0012
     terminator      1198..1238
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gaaatatgta acgttatacg atgtcgcaga gtatgccggt
       61 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
      121 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
      181 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
      241 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
      301 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
      361 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
      421 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
      481 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg
      541 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
      601 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
      661 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
      721 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
      781 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
      841 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
      901 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
      961 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
     1021 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     1081 cgactggaaa gcgggcagtg atactagagc caggcatcaa ataaaacgaa aggctcagtc
     1141 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1201 cactggctca ccttcgggtg ggcctttctg cgtttata
//