LOCUS       BBa_S04064              1276 bp    DNA     linear       26-Nov-2024
DEFINITION  K137048:S03998
ACCESSION   BBa_S04064
VERSION     BBa_S04064.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=tetR promoter
     promoter        1..350
                     /label=BBa_K137048
     misc_feature    359..375
                     /label=IRR out
     misc_feature    377..393
                     /label=IRR in
     misc_feature    359..393
                     /label=BBa_K137008
     misc_feature    406..409
                     /label=conserved
     RBS             402..413
                     /label=BBa_B0034
     CDS             420..1139
                     /label=GFP protein
     CDS             420..1139
                     /label=BBa_E0040
     stem_loop       1159..1202
                     /label=stem_loop
     misc_feature    1148..1227
                     /label=BBa_B0010
     terminator      1148..1227
                     /label=BBa_B0010
     stem_loop       1243..1262
                     /label=T7 TE
     misc_feature    1269..1269
                     /label=stop
     polyA_site      1263..1276
                     /label=polya
     misc_feature    1236..1276
                     /label=BBa_B0012
     terminator      1236..1276
                     /label=BBa_B0012
ORIGIN
        1 gtgctcagta tctctatcac tgatagggat gtcaatctct atcactgata gggactctag
       61 tatataaacg cagaaaggcc cacccgaagg tgagccagtg tgactctagt agagagcgtt
      121 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
      181 ttgatgcctg gctctagtag tgatctacac tagcactatc agtgttatta agctactaaa
      241 gcgtagtttt cgtcgtttgc agcggaccca ctttcacatt taagttgttt ttctaatccg
      301 catatgatca attcaaggcc gaataagaag gctggctctg caccttggtg tactagagaa
      361 gatgaaacat ttggggccaa actgtccata ttatactaga gaaagaggag aaatactaga
      421 tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
      481 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
      541 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
      601 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc
      661 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
      721 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta
      781 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
      841 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
      901 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc
      961 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     1021 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
     1081 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaataataat
     1141 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1201 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     1261 cctttctgcg tttata
//